PDF(Pubmed)
Abstract：
Houttuynia cordata Thunb is rich in active substances and has excellent antioxidant and anti-inflammatory activity. Scanning electron microscopy and gel permeation chromatography were used to analyze the molecular characteristics of the fermentation broth of Houttuynia cordata Thunb obtained through fermentation with Clavispora lusitaniae (HCT-f). The molecular weight of HCT-f was 2.64265 × 105 Da, and the polydispersity coefficient was 183.10, which were higher than that of unfermented broth of Houttuynia cordata Thunb (HCT). By investigating the active substance content and in vitro antioxidant activity of HCT-f and HCT, the results indicated that HCT-f had a higher active substance content and exhibited a superior scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radicals and hydroxyl radicals, with IC50 values of 11.85% and 9.01%, respectively. Our results showed that HCT-f could effectively alleviate the increase in the secretion of inflammatory factors and apoptotic factors caused by lipopolysaccharide (LPS) stimulation, and had a certain effect on repairing skin barrier damage. HCT-f could exert an anti-inflammatory effect by down-regulating signaling in the MAPK/NF-κB pathway. The results of erythrocyte hemolysis and chicken embryo experiments showed that HCT-f had a high safety profile. Therefore, this study provides a theoretical basis for the application of HCT-f as an effective ingredient in food and cosmetics.
摘要：
鱼腥草含有丰富的活性物质，具有优异的抗氧化和抗炎活性。采用扫描电子显微镜和凝胶渗透色谱法分析了鱼腥草（HCT-f）发酵获得的鱼腥草发酵液的分子特征。HCT-f的分子量为2.64265×105Da,鱼腥草的多分散系数为183.10，高于未发酵液（HCT）的多分散系数。通过研究HCT-f和HCT的活性物质含量和体外抗氧化活性，结果表明，HCT-f具有较高的活性物质含量，对2，2-二苯基-1-吡啶酰肼基和羟基自由基具有良好的清除作用，IC50值为11.85%和9.01%，分别。结果表明,HCT-f能有效缓解脂多糖(LPS)刺激引起的炎症因子和凋亡因子的分泌增加,对修复皮肤屏障损伤有一定的效果。HCT-f可能通过下调MAPK/NF-κB通路中的信号传导而发挥抗炎作用。红细胞溶血和鸡胚实验结果表明HCT-f具有较高的安全性。因此,本研究为HCT-f作为有效成分在食品和化妆品中的应用提供了理论依据。
