PDF(Pubmed)
Abstract：
Lemon is a fruit rich in antioxidant properties and has several health benefits, namely the reduction of skin edema and anticarcinogenic properties, which are due to its high content of bioactive compounds. Melatonin can improve and preserve the properties of lemon for longer and also has health benefits. The aim of this study was to evaluate the effects of oral administration of lemon juice after melatonin treatment on murinometric parameters of wild-type (WT) mice and transgenic mice carrying human papillomavirus (HPV). Two trials were performed for oral administration of the lemon extract compound: in drinking water and in diet. First of all, lemons were treated by immersion with melatonin at 10 mM. Then, lemons were squeezed, and the juice obtained was freeze-dried and stored to be subsequently added to drinking water or diet, according to the assay. Thus, mice were divided into eight groups in the drink assay (each with n = 5): group 1 (G1, WT, control), group 2 (G2, WT, 1 mL lemon), group 3 (G3, WT, 1.5 mL lemon), group 4 (G4, WT, 2 mL lemon), group 5 (G5, HPV16, control), group 6 (G6, HPV16, 1 mL lemon) group 7 (G6, HPV16, 1.5 mL lemon) and group 8 (G6, HPV16, 2 mL lemon). The diet assay was divided into four groups: group 1 (G1, WT, control), group 2 (G2, WT, 4 mL lemon), group 3 (G3, HPV16, control) and group 4 (G4, HPV16, 4 mL lemon). In the drink assay, the highest concentration of melatonin (308 ng/100 mL) was for groups 4 and 8, while in the food assay, there was only one concentration of melatonin (9.96 ng/g) for groups 2 and 4. Both trials lasted 30 days. During this time, body weight, food and water were recorded. Afterward, they were sacrificed, and samples were collected for different analyses. At the concentrations used, the lemon juice with melatonin had no adverse effects on the animals\' health and showed a positive outcome in modifying weight gain and enhancing antioxidant activity in mice. Moreover, a reduction in the incidence of histological lesions was observed in treated animals. Further research is needed to better understand the effects of lemon extract on health and treatment outcomes in this animal model.
摘要：
柠檬是一种富含抗氧化特性的水果，具有多种健康益处，即减少皮肤水肿和抗癌特性，这是由于其高含量的生物活性化合物。褪黑激素可以更长时间地改善和保存柠檬的特性，并且还具有健康益处。这项研究的目的是评估褪黑激素治疗后口服柠檬汁对野生型（WT）小鼠和携带人乳头瘤病毒（HPV）的转基因小鼠的小鼠测量参数的影响。对柠檬提取物化合物的口服给药进行了两项试验：在饮用水和饮食中。首先,柠檬用10mM褪黑激素浸泡处理。然后，柠檬被挤压，并将获得的果汁冷冻干燥并储存以随后添加到饮用水或饮食中，根据化验。因此，在饮料测定中将小鼠分为八组（每组n=5）：第1组（G1，WT，control),组2（G2，WT，1毫升柠檬)，组3(G3，WT，1.5毫升柠檬)，第4组(G4，WT，2毫升柠檬)，第5组（G5，HPV16，对照），第6组（G6，HPV16，1mL柠檬）第7组（G6，HPV16，1.5mL柠檬）和第8组（G6，HPV16，2mL柠檬）。饮食测定分为四组：第1组（G1，WT，control),组2（G2，WT，4毫升柠檬)，第3组（G3，HPV16，对照组）和第4组（G4，HPV16，4mL柠檬）。在饮料检测中，褪黑激素的最高浓度（308ng/100mL）是第4组和第8组，而在食物测定中，第2组和第4组只有一种褪黑素浓度(9.96ng/g).两次试验持续了30天。在此期间,体重,食物和水被记录下来。之后,他们被牺牲了，并收集样本进行不同的分析。在使用的浓度下，柠檬汁与褪黑激素对动物的健康没有不利影响，并且在改善小鼠体重增加和增强抗氧化活性方面显示出积极的结果。此外，在接受治疗的动物中观察到组织学损伤发生率的降低.需要进一步的研究来更好地了解柠檬提取物对这种动物模型的健康和治疗结果的影响。
