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Abstract：
Several medicinal herbal plants are extensively used as sources of bioactive compounds with beneficial effects on human health. This study assessed the procyanidin and polyphenol profiles together with the antioxidant potential of seven herbal medical matrices. To achieve this aim, procyanidin extraction from grape pomace was optimized and validated by monitoring monomeric-trimeric procyanidins. The proposed quantification method was applied to the seven medical herbs, and it proved to be a very efficient protocol for procyanidin-rich extracts analysis. In addition, the Paullinia cupana Kunth. seed was identified as a very rich source of procyanidins (about 5 mg/g dry matrix of each dimeric and about 3 mg/g dry matrix trimeric) with high antioxidant properties. The polyphenolic profile was assessed by HPLC-HESI-MS/MS analysis. The in vitro antioxidant activity was evaluated by DPPH assay to explore the antioxidant properties of the extracts, which were substantially higher in Peumus boldus Molina leaves extracts (935.23 ± 169 μmol of Trolox equivalent/g of dry weight) concerning the other matrices. Moreover, a high Pearson coefficient value was observed between the total flavonoid content (TFC) and DPPH in comparison with the total polyphenol content (TPC) and DPPH, indicating flavonoids as the principal bioactive with antioxidant activity in the extracts.
摘要：
几种草药植物被广泛用作对人类健康有益的生物活性化合物的来源。这项研究评估了原花青素和多酚的概况以及七种草药基质的抗氧化潜力。为了实现这一目标，通过监测单体三聚体原花青素，优化并验证了从葡萄皮渣中提取原花青素的方法。将所提出的定量方法应用于七种草药，它被证明是富含原花青素的提取物分析的非常有效的方案。此外,PaulliniacupanaKunth.种子被确定为具有高抗氧化性能的原花青素的非常丰富的来源（每个二聚体约5mg/g干基质和约3mg/g干基质三聚体）。通过HPLC-HESI-MS/MS分析评估多酚谱。通过DPPH测定法评估其体外抗氧化活性，以探索提取物的抗氧化性能，在其他基质中，PeumusboldusMolina叶提取物中的含量明显更高（Trolox当量为935.23±169μmol/g干重）。此外，与总多酚含量（TPC）和DPPH相比，总黄酮含量（TFC）和DPPH之间观察到较高的皮尔逊系数值，表明黄酮是提取物中具有抗氧化活性的主要生物活性物质。
