PDF(Pubmed)
Abstract：
The application of Neurospora sp., a fungus that commonly thrives on complex agricultural and plant wastes, has proven successful in utilizing citrus peel waste as a source of naringin. A UV-Vis spectrophotometric method proved the biotransformation of naringin, with an absorption maximum (λmax) observed at 310 nm for the biotransformed product, naringenin (NAR). Further verification of the conversion of naringin was provided through thin layer chromatography (TLC). The Neurospora crassa mediated biotransformation of naringin to NAR was utilized for the rapid (within 5 min) synthesis of silver (Ag) and gold (Au) nanoconjugates using sunlight to accelerate the reaction. The synthesized NAR-nano Ag and NAR-nano Au conjugates exhibited monodispersed spherical and spherical as well as polygonal shaped particles, respectively. Both of the nanoconjugates showed average particle sizes of less than 90 nm from TEM analysis. The NAR-Ag and NAR-Au nanoconjugates displayed potential enhancement of the antimicrobial activities, including antibacterial and nematicidal properties over either standalone NAR or Ag or Au NPs. This study reveals the potential of naringinase-producing Neurospora sp. for transforming naringin into NAR. Additionally, the resulting NAR-Ag and NAR-Au nanoconjugates showed promise as sustainable antibiotics and biochemical nematicides.
摘要：
神经孢菌的应用。,一种真菌，通常在复杂的农业和植物废物中生长，已被证明成功地利用柑橘皮废物作为柚皮苷的来源。紫外可见分光光度法证明了柚皮苷的生物转化，在310nm处观察到生物转化产物的吸收最大值（λmax），柚皮苷(NAR)。通过薄层色谱(TLC)提供柚皮苷转化的进一步验证。利用Neurosporacrassa介导的柚皮苷向NAR的生物转化，利用阳光加速反应，快速（在5分钟内）合成银（Ag）和金（Au）纳米缀合物。合成的NAR-纳米Ag和NAR-纳米Au共轭物表现出单分散的球形和球形以及多边形颗粒，分别。根据TEM分析,两种纳米缀合物显示小于90nm的平均粒度。NAR-Ag和NAR-Au纳米共轭物显示抗菌活性的潜在增强，包括独立NAR或Ag或AuNP的抗菌和杀线虫特性。这项研究揭示了产生柚皮苷酶的神经孢菌的潜力。将柚皮苷转化为NAR。此外,所得的NAR-Ag和NAR-Au纳米缀合物显示出作为可持续抗生素和生化杀线虫剂的前景。
