PDF(Pubmed)
Abstract：
Hydrolyzed royal jelly peptide (RJP) has garnered attention for its health-promoting functions. However, the potential applications of RJP in skincare have not been fully explored. In this study, we prepared RJP through the enzymatic hydrolysis of royal jelly protein with trypsin and investigated its antioxidant and anti-inflammatory properties on primary human dermal fibroblasts (HDFs). Our results demonstrate that RJP effectively inhibits oxidative damage induced by H2O2 and lipid peroxidation triggered by AAPH and t-BuOOH in HDFs. This effect may be attributed to the ability of RJP to enhance the level of glutathione and the activities of catalase and glutathione peroxidase 4, as well as its excellent iron chelating capacity. Furthermore, RJP modulates the NLRP3 inflammasome-mediated inflammatory response in HDFs, suppressing the mRNA expressions of NLRP3 and IL-1β in the primer stage induced by LPS and the release of mature IL-1β induced by ATP, monosodium urate, or nigericin in the activation stage. RJP also represses the expressions of COX2 and iNOS induced by LPS. Finally, we reveal that RJP exhibits superior antioxidant and anti-inflammatory properties over unhydrolyzed royal jelly protein. These findings suggest that RJP exerts protective effects on skin cells through antioxidative and anti-inflammatory mechanisms, indicating its promise for potential therapeutic avenues for managing oxidative stress and inflammation-related skin disorders.
摘要：
水解蜂王浆肽（RJP）因其促进健康的功能而受到关注。然而,RJP在护肤中的潜在应用尚未得到充分探索。在这项研究中,我们通过胰蛋白酶酶水解蜂王浆蛋白制备RJP，并研究其对原代人真皮成纤维细胞（HDFs）的抗氧化和抗炎特性。我们的结果表明，RJP有效抑制HDFs中H2O2诱导的氧化损伤和AAPH和t-BuOOH引发的脂质过氧化。这种作用可能归因于RJP增强谷胱甘肽水平以及过氧化氢酶和谷胱甘肽过氧化物酶4活性的能力，以及其出色的铁螯合能力。此外，RJP调节HDFs中NLRP3炎性小体介导的炎症反应,抑制LPS诱导的引物期NLRP3和IL-1β的mRNA表达，抑制ATP诱导的成熟IL-1β的释放，尿酸单钠,或尼格林处于激活阶段。RJP还抑制LPS诱导的COX2和iNOS的表达。最后,我们发现，RJP表现出优于未水解蜂王浆蛋白的抗氧化和抗炎性能。这些发现表明RJP通过抗氧化和抗炎机制对皮肤细胞发挥保护作用。这表明它有望成为治疗氧化应激和炎症相关皮肤病的潜在治疗途径。
