PDF(Pubmed)
Abstract：
There is a growing interest in the production of bioinks that on the one hand, are biocompatible and, on the other hand, have mechanical properties that allow for the production of stable constructs that can survive for a long time after transplantation. While the selection of the right material is crucial for bioprinting, there is another equally important issue that is currently being extensively researched-the incorporation of the vascular system into the fabricated scaffolds. Therefore, in the following manuscript, we present the results of research on bioink with unique physico-chemical and biological properties. In this article, two methods of seeding cells were tested using bioink B and seeding after bioprinting the whole model. After 2, 5, 8, or 24 h of incubation, the flow medium was used in the tested systems. At the end of the experimental trial, for each time variant, the canals were stored in formaldehyde, and immunohistochemical staining was performed to examine the presence of cells on the canal walls and roof. Cells adhered to both ways of fiber arrangement; however, a parallel bioprint with the 5 h incubation and the intermediate plating of cells resulted in better adhesion efficiency. For this test variant, the percentage of cells that adhered was at least 20% higher than in the other analyzed variants. In addition, it was for this variant that the lowest percentage of viable cells was found that were washed out of the tested model. Importantly, hematoxylin and eosin staining showed that after 8 days of culture, the cells were evenly distributed throughout the canal roof. Our study clearly shows that neovascularization-promoting cells effectively adhere to ECM-based pancreatic bioink. Summarizing the presented results, it was demonstrated that the proposed bioink compositions can be used for bioprinting bionic organs with a vascular system formed by endothelial cells and fibroblasts.
摘要：
人们对生物墨水的生产越来越感兴趣，一方面，是生物相容的，另一方面,具有机械性能，可以生产出稳定的构建体，这些构建体在移植后可以长时间存活。虽然选择正确的材料对于生物打印至关重要，还有另一个同样重要的问题，目前正在广泛研究-血管系统的结合到制造的支架。因此,在下面的手稿中，我们提出了具有独特物理化学和生物学特性的生物墨水的研究结果。在这篇文章中,使用生物墨水B测试两种接种细胞的方法，并在生物打印整个模型后进行接种。孵育2、5、8或24小时后，在测试系统中使用流动介质。在实验试验结束时,对于每个时间变体，运河储存在甲醛中，进行免疫组织化学染色以检查管壁和屋顶上细胞的存在。细胞粘附于两种纤维排列方式；然而，与5小时孵育和细胞中间铺板的平行生物打印导致更好的粘附效率。对于此测试变体，粘附的细胞百分比比其他分析的变体高至少20%。此外,对于这种变体，发现从测试模型中洗掉的最低百分比的活细胞。重要的是,苏木精和伊红染色显示培养8天后,细胞均匀分布在整个运河屋顶。我们的研究清楚地表明，促进新生血管形成的细胞有效地粘附于基于ECM的胰腺生物墨水。总结提出的结果，已证明，所提出的生物墨水组合物可用于生物打印具有由内皮细胞和成纤维细胞形成的血管系统的仿生器官。
