PDF(Pubmed)
Abstract：
Pistacia lentiscus var. chia is a valuable crop for its high-added-value mastic, a resin with proven pharmaceutical and cosmeceutical properties harvested from the male tree trunk. To achieve the maximum economic benefits from the cultivation of male mastic trees, it is important to develop early sex diagnosis molecular tools for distinguishing the sex type. Thus far, the work on sex identification has focused on Pistacia vera with promising results; however, the low transferability rates of these markers in P. lentiscus necessitates the development of species-specific sex-linked markers for P. lentiscus var. chia. To our knowledge, this is the first report regarding: (i) the development of species-specific novel transcriptome-based markers for P. lentiscus var. chia and their assessment on male, female and monoecious individuals using PCR-HRM analysis, thus, introducing a cost-effective method for sex identification with high accuracy that can be applied with minimum infrastructure, (ii) the effective sex identification in mastic tree using a combination of different sex-linked ISSR and SCAR markers with 100% accuracy, and (iii) the impact evaluation of sex type on the genetic diversity of different P. lentiscus var. chia cultivars. The results of this study are expected to provide species-specific markers for accurate sex identification that could contribute to the selection process of male mastic trees at an early stage for mass propagation systems and to facilitate future breeding efforts related to sex-linked productivity and quality of mastic resin.
摘要：
Pistacialentiscusvar.正大是一种有价值的高附加值乳香作物，一种从雄性树干上收获的具有经过验证的药物和药妆特性的树脂。为了从雄性乳香树的种植中获得最大的经济效益，开发早期性别诊断分子工具对区分性别类型很重要。到目前为止,性别鉴定工作的重点是Pistaciavera，取得了可喜的成果；然而，这些标记在猪扁桃体中的可转移性较低，因此有必要开发针对猪扁桃体的物种特异性性别连锁标记。chia.据我们所知,这是有关以下方面的第一份报告：（i）针对P.lentiscusvar的基于物种特异性转录组的新型标记的开发。chia和他们对男性的评估，使用PCR-HRM分析的女性和雌雄同株的个体，因此，引入一种具有成本效益的高精度性别识别方法，该方法可以在最少的基础设施下应用，（ii）使用不同的性别连锁ISSR和SCAR标记组合以100％的准确性对乳香树进行有效的性别鉴定，（iii）性别类型对不同品系的遗传多样性的影响评价。chia品种。这项研究的结果有望为准确的性别鉴定提供物种特异性标记，这可能有助于大规模繁殖系统早期选择雄性乳香树的过程，并促进与性别相关的生产力和质量相关的未来育种工作。乳香树脂。
