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Abstract：
Soybean mosaic virus (SMV) is one of the main pathogens that can negatively affect soybean production and quality. To study the gene regulatory network of soybeans in response to SMV SC15, the resistant line X149 and susceptible line X97 were subjected to transcriptome analysis at 0, 2, 8, 12, 24, and 48 h post-inoculation (hpi). Differential expression analysis revealed that 10,190 differentially expressed genes (DEGs) responded to SC15 infection. Weighted gene co-expression network analysis (WGCNA) was performed to identify highly related resistance gene modules; in total, eight modules, including 2256 DEGs, were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of 2256 DEGs revealed that the genes significantly clustered into resistance-related pathways, such as the plant-pathogen interaction pathway, mitogen-activated protein kinases (MAPK) signaling pathway, and plant hormone signal transduction pathway. Among these pathways, we found that the flg22, Ca2+, hydrogen peroxide (H2O2), and abscisic acid (ABA) regulatory pathways were fully covered by 36 DEGs. Among the 36 DEGs, the gene Glyma.01G225100 (protein phosphatase 2C, PP2C) in the ABA regulatory pathway, the gene Glyma.16G031900 (WRKY transcription factor 22, WRKY22) in Ca2+ and H2O2 regulatory pathways, and the gene Glyma.04G175300 (calcium-dependent protein kinase, CDPK) in Ca2+ regulatory pathways were highly connected hub genes. These results indicate that the resistance of X149 to SC15 may depend on the positive regulation of flg22, Ca2+, H2O2, and ABA regulatory pathways. Our study further showed that superoxide dismutase (SOD) activity, H2O2 content, and catalase (CAT) and peroxidase (POD) activities were significantly up-regulated in the resistant line X149 compared with those in 0 hpi. This finding indicates that the H2O2 regulatory pathway might be dependent on flg22- and Ca2+-pathway-induced ROS generation. In addition, two hub genes, Glyma.07G190100 (encoding F-box protein) and Glyma.12G185400 (encoding calmodulin-like proteins, CMLs), were also identified and they could positively regulate X149 resistance. This study provides pathways for further investigation of SMV resistance mechanisms in soybean.
摘要：
大豆花叶病毒（SMV）是影响大豆生产和品质的主要病原体之一。为了研究大豆响应SMVSC15的基因调控网络,在接种后0、2、8、12、24和48小时(hpi)对抗性系X149和易感系X97进行转录组分析。差异表达分析显示，10,190个差异表达基因（DEGs）对SC15感染有反应。进行加权基因共表达网络分析(WGCNA)以鉴定高度相关的抗性基因模块;八个模块，包括2256个DEG，已确定。对2256DEGG的京都基因和基因组百科全书（KEGG）途径富集分析显示，这些基因显着聚集在抗性相关途径中，例如植物-病原体相互作用途径，丝裂原活化蛋白激酶(MAPK)信号通路,和植物激素信号转导途径。在这些途径中，我们发现flg22，Ca2+，过氧化氢(H2O2),36个DEG完全覆盖了脱落酸（ABA）调节途径。在36个DEG中，基因Glyma.01G225100(蛋白磷酸酶2C，PP2C）在ABA调节途径中，基因Glyma.16G031900(WRKY转录因子22,WRKY22)在Ca2+和H2O2调控途径中,和基因Glyma.04G175300（钙依赖性蛋白激酶，CDPK)中Ca2+调控途径是高度连接的hub基因。这些结果表明，X149对SC15的抗性可能取决于flg22，Ca2，H2O2和ABA调控途径。我们的研究进一步表明，超氧化物歧化酶（SOD）活性，H2O2含量,与0hpi相比，抗性品系X149中的过氧化氢酶（CAT）和过氧化物酶（POD）活性显着上调。这一发现表明H2O2调节途径可能依赖于flg22-和Ca2+途径诱导的ROS产生。此外,两个枢纽基因，Glyma.07G190100（编码F-box蛋白）和Glyma.12G185400（编码钙调蛋白样蛋白，CMLs),还鉴定了它们，它们可以正向调节X149抗性。本研究为进一步研究大豆SMV抗性机制提供了途径。
