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Abstract：
BACKGROUND: This study aimed to evaluate dentin wear and biological performance of desensitizing materials.
METHODS: Seventy bovine root dentin blocks were sectioned. Half of the surface of each specimen was untreated (control) and the other half was immersed in EDTA and treated with the following desensitizing materials: placebo varnish (PLA), fluoride varnish (FLU), sodium fluoride (NaF) varnish + sodium trimetaphosphate (TMP), universal adhesive (SBU), S-PRG varnish (SPRG), biosilicate (BIOS), and amelotin solution (AMTN). After application, the specimens were submitted to an erosive-abrasive challenge and the wear analyzed by optical profilometer. Serial dilutions of extracts obtained from the culture medium containing discs impregnated with those desensitizers were applied on fibroblasts and odontoblasts-like cells cultures. Cytotoxicity and production of total protein (TP) by colorimetric assays were determined after 24 h. Data were statistically analyzed using Kruskal-Wallis, Dunn\'s, One-way ANOVA and Tukey tests (p ≤ 0.05).
RESULTS: No dentin wear was observed only for SBU. The lowest dentin wear was observed for AMTN and TMP. Cell viability was significantly reduced after treatment with undiluted extracts of PLA, FLU, TMP and SBU in fibroblasts and TMP and SBU in odontoblast-like cells. SPRG, BIOS and AMTN were cytocompatible at all dilutions tested. Considering TP results, no statistical difference was observed among the groups and high levels for TP were observed after TMP and FLU treatments.
CONCLUSIONS: Universal adhesive system may protect dentin with opened tubules from wear after challenge. Extracts of adhesive and fluoride varnishes presented cytotoxic mainly on fibroblasts. The enamel protein may be a future alternative to treat dentin with opened tubules because it may cause low wear under erosive-abrasive challenge with low cytotoxic effects.
METHODS: Seventy bovine root dentin blocks were sectioned. Half of the surface of each specimen was untreated (control) and the other half was immersed in EDTA and treated with the following desensitizing materials: placebo varnish (PLA), fluoride varnish (FLU), sodium fluoride (NaF) varnish + sodium trimetaphosphate (TMP), universal adhesive (SBU), S-PRG varnish (SPRG), biosilicate (BIOS), and amelotin solution (AMTN). After application, the specimens were submitted to an erosive-abrasive challenge and the wear analyzed by optical profilometer. Serial dilutions of extracts obtained from the culture medium containing discs impregnated with those desensitizers were applied on fibroblasts and odontoblasts-like cells cultures. Cytotoxicity and production of total protein (TP) by colorimetric assays were determined after 24 h. Data were statistically analyzed using Kruskal-Wallis, Dunn\'s, One-way ANOVA and Tukey tests (p ≤ 0.05).
RESULTS: No dentin wear was observed only for SBU. The lowest dentin wear was observed for AMTN and TMP. Cell viability was significantly reduced after treatment with undiluted extracts of PLA, FLU, TMP and SBU in fibroblasts and TMP and SBU in odontoblast-like cells. SPRG, BIOS and AMTN were cytocompatible at all dilutions tested. Considering TP results, no statistical difference was observed among the groups and high levels for TP were observed after TMP and FLU treatments.
CONCLUSIONS: Universal adhesive system may protect dentin with opened tubules from wear after challenge. Extracts of adhesive and fluoride varnishes presented cytotoxic mainly on fibroblasts. The enamel protein may be a future alternative to treat dentin with opened tubules because it may cause low wear under erosive-abrasive challenge with low cytotoxic effects.
摘要：
背景：本研究旨在评估脱敏材料的牙本质磨损和生物学性能。
方法：对70个牛根牙本质块进行切片。每个样本的一半表面未处理（对照），另一半浸入EDTA中并用以下脱敏材料处理：安慰剂清漆（PLA），氟化物清漆(FLU),氟化钠（NaF）清漆+三偏磷酸钠（TMP），通用胶粘剂(SBU)，S-PRG清漆(SPRG),生物硅酸盐(BIOS),和Amelotin溶液（AMTN）。申请后,样品被提交到一个侵蚀性的磨料挑战和磨损的光学轮廓仪分析。将从含有用那些脱敏剂浸渍的圆盘的培养基中获得的提取物的连续稀释液应用于成纤维细胞和成牙本质细胞样细胞培养物。24小时后通过比色法测定细胞毒性和总蛋白（TP）的产生。数据使用Kruskal-Wallis进行统计分析，邓恩，单因素方差分析和Tukey检验(p≤0.05)。
结果:仅对于SBU没有观察到牙本质磨损。对于AMTN和TMP,观察到最低的牙本质磨损。用未稀释的PLA提取物处理后，细胞活力显着降低，FLU,成纤维细胞中的TMP和SBU以及成牙本质细胞样细胞中的TMP和SBU。SPRG,BIOS和AMTN在所有测试的稀释度下是细胞相容的。考虑到TP结果，各组间无统计学差异,TMP和FLU治疗后TP水平升高.
结论：通用粘合剂系统可以保护牙本质与开放小管在挑战后的磨损。粘合剂和氟化物清漆的提取物主要对成纤维细胞具有细胞毒性。牙釉质蛋白可能是用开放小管治疗牙本质的未来替代品，因为它可能会在具有低细胞毒性作用的侵蚀磨蚀性挑战下引起低磨损。
方法：对70个牛根牙本质块进行切片。每个样本的一半表面未处理（对照），另一半浸入EDTA中并用以下脱敏材料处理：安慰剂清漆（PLA），氟化物清漆(FLU),氟化钠（NaF）清漆+三偏磷酸钠（TMP），通用胶粘剂(SBU)，S-PRG清漆(SPRG),生物硅酸盐(BIOS),和Amelotin溶液（AMTN）。申请后,样品被提交到一个侵蚀性的磨料挑战和磨损的光学轮廓仪分析。将从含有用那些脱敏剂浸渍的圆盘的培养基中获得的提取物的连续稀释液应用于成纤维细胞和成牙本质细胞样细胞培养物。24小时后通过比色法测定细胞毒性和总蛋白（TP）的产生。数据使用Kruskal-Wallis进行统计分析，邓恩，单因素方差分析和Tukey检验(p≤0.05)。
结果:仅对于SBU没有观察到牙本质磨损。对于AMTN和TMP,观察到最低的牙本质磨损。用未稀释的PLA提取物处理后，细胞活力显着降低，FLU,成纤维细胞中的TMP和SBU以及成牙本质细胞样细胞中的TMP和SBU。SPRG,BIOS和AMTN在所有测试的稀释度下是细胞相容的。考虑到TP结果，各组间无统计学差异,TMP和FLU治疗后TP水平升高.
结论：通用粘合剂系统可以保护牙本质与开放小管在挑战后的磨损。粘合剂和氟化物清漆的提取物主要对成纤维细胞具有细胞毒性。牙釉质蛋白可能是用开放小管治疗牙本质的未来替代品，因为它可能会在具有低细胞毒性作用的侵蚀磨蚀性挑战下引起低磨损。
