Abstract：
Rice husks are rich in xylan, which can be hydrolyzed by xylanase to form xylooligosaccharides (XOS). XOS are a functional oligosaccharide such as improving gut microbiota and antioxidant properties. In this study, the structure and functional characteristics of XOS were studied. The optimal xylanase hydrolysis conditions through response surface methodology (RSM) were: xylanase dosage of 3000 U/g, hydrolysis time of 3 h, hydrolysis temperature of 50 °C. Under this condition, the yield of XOS was 150.9 mg/g. The TG-DTG curve showed that XOS began to decompose at around 200 °C. When the concentration of XOS reached 1.0 g/L, the clearance rate of DPPH reached 65.76 %, and the scavenging rate of OH reached 62.10 %, while the clearance rate of ABTS free radicals reached 97.70 %, which was equivalent to the clearance rate of VC. XOS had a proliferative effect on four probiotics: Lactobacillus plantarum, Lactobacillus brucelli, Lactobacillus acidophilus, and Lactobacillus rhamnosus. However, the further experiments are needed to explore the improvement effect of XOS on human gut microbiota, laying a foundation for the effective utilization of XOS. XOS have a wide range of sources, low price, and broad development prospects. The reasonable utilization of XOS can bring greater economic benefits.
摘要：
稻壳富含木聚糖,可以通过木聚糖酶水解形成低聚木糖(XOS)。XOS是一种功能性寡糖，例如改善肠道微生物群和抗氧化特性。在这项研究中,研究了XOS的结构和功能特性。通过响应面法（RSM）确定木聚糖酶的最佳水解条件为：木聚糖酶用量为3000U/g，水解时间3小时，水解温度50℃。在这种情况下,XOS的产量为150.9mg/g。TG-DTG曲线显示XOS在200°C左右开始分解。当XOS浓度达到1.0g/L时,DPPH的清除率达到65.76%,OH的清除率达到62.10%，ABTS自由基的清除率达到97.70%,相当于VC的清除率。XOS对四种益生菌有增殖作用：植物乳杆菌，布鲁切乳杆菌,嗜酸乳杆菌，和鼠李糖乳杆菌.然而,需要进一步的实验来探索XOS对人体肠道菌群的改善作用，为XOS的有效利用奠定基础。XOS有广泛的来源，价格低廉,和广阔的发展前景。合理利用XOS可以带来更大的经济效益。
