Abstract：
BACKGROUND: Bioscaffolds and cells are two main components in the regeneration of damaged tissues via cell therapy. Umbilical cord stem cells are among the most well-known cell types for this purpose. The main objective of the present study was to evaluate the effect of the pretreatment of the foreskin acellular matrix (FAM) by monophosphoryl lipid A (MPLA) and Lactobacillus casei supernatant (LCS) on the attraction of human umbilical cord mesenchymal stem cells (hucMSC).
RESULTS: The expression of certain cell migration genes was studied using qRT-PCR. In addition to cell migration, transdifferentiation of these cells to the epidermal-like cells was evaluated via immunohistochemistry (IHC) and immunocytochemistry (ICC) of cytokeratin 19 (CK19). The hucMSC showed more tissue tropism in the presence of MPLA and LCS pretreated FAM compared to the untreated control group. We confirmed this result by scanning electron microscopy (SEM) analysis, glycosaminoglycan (GAG), collagen, and DNA content. Furthermore, IHC and ICC data demonstrated that both treatments increase the protein expression level of CK19.
CONCLUSIONS: Pretreatment of acellular bioscaffolds by MPLA or LCS can increase the migration rate of cells and also transdifferentiation of hucMSC to epidermal-like cells without growth factors. This strategy suggests a new approach in regenerative medicine.
RESULTS: The expression of certain cell migration genes was studied using qRT-PCR. In addition to cell migration, transdifferentiation of these cells to the epidermal-like cells was evaluated via immunohistochemistry (IHC) and immunocytochemistry (ICC) of cytokeratin 19 (CK19). The hucMSC showed more tissue tropism in the presence of MPLA and LCS pretreated FAM compared to the untreated control group. We confirmed this result by scanning electron microscopy (SEM) analysis, glycosaminoglycan (GAG), collagen, and DNA content. Furthermore, IHC and ICC data demonstrated that both treatments increase the protein expression level of CK19.
CONCLUSIONS: Pretreatment of acellular bioscaffolds by MPLA or LCS can increase the migration rate of cells and also transdifferentiation of hucMSC to epidermal-like cells without growth factors. This strategy suggests a new approach in regenerative medicine.
摘要：
背景：生物支架和细胞是通过细胞疗法再生受损组织的两个主要组成部分。脐带干细胞是用于此目的的最众所周知的细胞类型之一。本研究的主要目的是评估单磷酰脂质A（MPLA）和干酪乳杆菌上清液（LCS）预处理包皮无细胞基质（FAM）对人脐带间充质干细胞（hucMSC）的吸引力。
结果:使用qRT-PCR研究某些细胞迁移基因的表达。除了细胞迁移,通过细胞角蛋白19（CK19）的免疫组织化学（IHC）和免疫细胞化学（ICC）评估了这些细胞向表皮样细胞的转分化。与未处理的对照组相比，在MPLA和LCS预处理的FAM存在下，hucMSC显示出更多的组织嗜性。我们通过扫描电子显微镜（SEM）分析证实了这一结果，糖胺聚糖（GAG），胶原蛋白，和DNA含量。此外，IHC和ICC数据表明两种处理都增加了CK19的蛋白质表达水平。
结论：通过MPLA或LCS预处理无细胞生物支架可以增加细胞的迁移率，也可以增加hucMSC向不含生长因子的表皮样细胞的转分化。这一策略提出了再生医学的新方法。
结果:使用qRT-PCR研究某些细胞迁移基因的表达。除了细胞迁移,通过细胞角蛋白19（CK19）的免疫组织化学（IHC）和免疫细胞化学（ICC）评估了这些细胞向表皮样细胞的转分化。与未处理的对照组相比，在MPLA和LCS预处理的FAM存在下，hucMSC显示出更多的组织嗜性。我们通过扫描电子显微镜（SEM）分析证实了这一结果，糖胺聚糖（GAG），胶原蛋白，和DNA含量。此外，IHC和ICC数据表明两种处理都增加了CK19的蛋白质表达水平。
结论：通过MPLA或LCS预处理无细胞生物支架可以增加细胞的迁移率，也可以增加hucMSC向不含生长因子的表皮样细胞的转分化。这一策略提出了再生医学的新方法。
