PDF(Pubmed)
Abstract：
The present study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from the nasal swabs of goats. A total of 232 nasal samples (one per animal) were collected from goats on 13 farms located in two regions of Algeria and were analyzed for the presence of S. aureus. The detection of virulence factors was carried out using PCR. The antibiotic susceptibility of the recovered isolates was assessed using the disc diffusion method. The biofilm formation ability was assessed by the Congo red agar method and a microtiter plate assay, and the molecular characterization of isolates was carried out by spa-typing, and for selected isolates also by multilocus sequence typing (MLST). Overall, 36 out of 232 nasal swabs (15.5%) contained S. aureus, and 62 isolates were recovered. Regarding the virulence factors, at least one staphylococcal enterotoxin gene was detected in 30 (48.4%) isolates. The gene tst encoding the toxic shock syndrome toxin was detected in fifteen isolates (24.2%), but none of the isolates harbored the gene of Panton-Valentine leukocidin (lukF/S-PV). Nine different spa-types were identified, including the detection of a new one (t21230). The recovered isolates were assigned to three clonal complexes, with CC5 (51.8%) being the most common lineage. Two isolates were methicillin-resistant (MRSA) and belonged to ST5 (CC5) and to spa-types t450 and t688. Moreover, 27 (43.5%) of the S. aureus isolates were found to be slime producers in Congo red agar, and all of the recovered isolates could produce biofilms in the microtiter plate assay. Our study showed that the nares of healthy goats could be a reservoir of toxigenic and antibiotic-resistant strains of S. aureus isolates, including MRSA, which could have implications for public health.
摘要：
本研究旨在确定从山羊鼻拭子中分离出的金黄色葡萄球菌的表型和基因型特征。从位于阿尔及利亚两个地区的13个农场的山羊收集总共232个鼻样品(每只动物一个),并分析金黄色葡萄球菌的存在。使用PCR进行毒力因子的检测。使用圆盘扩散法评估回收的分离株的抗生素敏感性。通过刚果红琼脂方法和微量滴定板测定法评估生物膜形成能力，分离株的分子特征是通过spa分型进行的，并通过多位点序列分型（MLST）选择分离株。总的来说,232个鼻拭子中有36个（15.5%）含有金黄色葡萄球菌，并回收了62个分离株。关于毒力因子，在30个(48.4%)分离株中检测到至少一个葡萄球菌肠毒素基因.在15个分离株中检测到编码中毒性休克综合征毒素的基因tst(24.2%),但是没有一个分离株带有Panton-Valentine杀白细胞素（lukF/S-PV）的基因。确定了九种不同的温泉类型，包括检测新的(t21230)。回收的分离株被分配到三个克隆复合物中，CC5（51.8%）是最常见的谱系。两个分离株是耐甲氧西林（MRSA），属于ST5（CC5）和spa型t450和t688。此外，在刚果红琼脂中发现27（43.5％）金黄色葡萄球菌分离物是粘液生产者，在微量滴定板测定中，所有回收的分离株都能产生生物膜。我们的研究表明，健康山羊的鼻孔可能是金黄色葡萄球菌分离株的产毒和抗生素抗性菌株的储库，包括MRSA,这可能会对公众健康产生影响。
