PDF(Pubmed)
Abstract：
2-naphthylamine (NAP) was classified as a group I carcinogen associated with bladder cancer. The daily exposure is mostly from cigarette and E-cigarette smoke. NAP can lead to testicular atrophy and interstitial tissue hyperplasia; however, the outcomes of NAP treatment on spermatogenesis and the associated mechanisms have not been reported. The study aimed to investigate the effect of NAP on spermatogenesis and sperm physiologic functions after being persistently exposed to NAP at 5, 20, and 40 mg/kg for 35 days. We found that sperm motility, progressive motility, sperm average path velocity, and straight-line velocity declined remarkably in the NAP (40 mg/kg) treated group, and the sperm deformation rate rose upon NAP administration. The testis immunity- and lipid metabolism-associated processes were enriched from RNA-sequence profiling. Plvap, Ccr7, Foxn1, Trim29, Sirpb1c, Cfd, and Lpar4 involved in testis immunity and Pnliprp1 that inhibit triglyceride and cholesterol absorption were confirmed to rise dramatically in the NAP-exposed group. The increased total cholesterol and CD68 levels were observed in the testis from the NAP-exposed group. Gpx5, serving as an antioxidant in sperm plasma, and Semg1, which contributes to sperm progressive motility, were both down-regulated. We concluded that the short-term exposure to NAP caused reproductive toxicity, primarily due to the inflammatory abnormality in the testis.
摘要：
2-萘胺（NAP）被归类为与膀胱癌相关的I组致癌物。日常接触主要来自香烟和电子烟烟雾。NAP可导致睾丸萎缩和间质组织增生；然而，NAP治疗对精子发生的影响及相关机制尚未见报道.该研究旨在研究持续暴露于5、20和40mg/kg的NAP35天后，NAP对精子生成和精子生理功能的影响。我们发现精子的运动性,渐进运动，精子平均路径速度，NAP（40mg/kg）治疗组的直线速度显着下降，施用NAP后，精子变形率上升。从RNA序列谱分析中富集了睾丸免疫和脂质代谢相关的过程。Plvap,Ccr7,Foxn1,Trim29,Sirpb1c,cfd,在NAP暴露组中，参与睾丸免疫的Lpar4和抑制甘油三酯和胆固醇吸收的Pnliprp1被证实急剧上升。在NAP暴露组的睾丸中观察到总胆固醇和CD68水平升高。Gpx5，作为精子血浆中的抗氧化剂，和Semg1，它有助于精子的进行性运动，都被下调了。我们得出结论，短期暴露于NAP会导致生殖毒性，主要是由于睾丸的炎症异常。
