PDF(Pubmed)
Abstract：
Insects constitute the largest proportion of animals on Earth and act as significant reservoirs and vectors in disease transmission. Rice thrips (Haplothrips aculeatus, family Phlaeothripidae) are one of the most common pests in agriculture. In this study, the full genome sequence of a novel Ollusvirus, provisionally named \"Rice thrips ollusvirus 1\" (RTOV1), was elucidated using transcriptome sequencing and the rapid amplification of cDNA ends (RACE). A homology search and phylogenetic tree analysis revealed that the newly identified virus is a member of the family Aliusviridae (order Jingchuvirales). The genome of RTOV1 contains four predicted open reading frames (ORFs), including a polymerase protein (L, 7590 nt), a glycoprotein (G, 4206 nt), a nucleocapsid protein (N, 2415 nt) and a small protein of unknown function (291 nt). All of the ORFs are encoded by the complementary genome, suggesting that the virus is a negative-stranded RNA virus. Phylogenetic analysis using polymerase sequences suggested that RTOV1 was closely related to ollusvirus 1. Deep small RNA sequencing analysis reveals a significant accumulation of small RNAs derived from RTOV1, indicating that the virus replicated in the insect. According to our understanding, this is the first report of an Ollusvirus identified in a member of the insect family Phlaeothripidae. The characterisation and discovery of RTOV1 is a significant contribution to the understanding of Ollusvirus diversity in insects.
摘要：
昆虫占地球上动物的最大比例，是疾病传播的重要水库和媒介。水稻蓟马（Haplothripsaculeatus，Phlaeothripidae）是农业中最常见的害虫之一。在这项研究中,一种新型Ollus病毒的全基因组序列,暂时命名为“水稻蓟马-1型病毒”(RTOV1)，使用转录组测序和cDNA末端的快速扩增（RACE）进行了阐明。同源性搜索和系统发育树分析表明，新鉴定的病毒是Aliusviridae家族（Jingchuvirales目）的成员。RTOV1的基因组包含四个预测的开放阅读框（ORF），包括聚合酶蛋白(L，7590nt)，糖蛋白(G，4206nt),一种核衣壳蛋白(N，2415nt）和功能未知的小蛋白（291nt）。所有的ORF都是由互补基因组编码的,这表明该病毒是一种负链RNA病毒。使用聚合酶序列进行的系统发育分析表明，RTOV1与1型流感病毒密切相关。深度小RNA测序分析揭示源自RTOV1的小RNA的显著积累,表明病毒在昆虫中复制。根据我们的理解,这是在昆虫科Phlaeothripidae成员中鉴定出的Ollus病毒的第一份报告。RTOV1的表征和发现对理解昆虫中的Ollus病毒多样性做出了重要贡献。
