PDF(Pubmed)
Abstract：
Plasma volume (PV) undergoes constant and dynamic changes, leading to a large intra-day variability in healthy individuals. Hydration is known to induce PV changes; however, the response to the intake of osmotically different fluids is still not fully understood. In a randomized controlled crossover trial, 18 healthy individuals (10 females) orally received an individual amount of an isotonic sodium-chloride (ISO), Ringer (RIN), or glucose (GLU) solution. Hemoglobin mass (Hbmass) was determined with the optimized carbon monoxide re-breathing method. Fluid-induced changes in PV were subsequently calculated based on capillary hemoglobin concentration ([Hb]) and hematocrit (Hct) before and then every 10 minutes until 120 min (t0-120) after the fluid intake and compared to a control trial arm (CON), where no fluid was administered. Within GLU and CON trial arms, no statistically significant differences from baseline until t120 were found (p > 0.05). In the ISO trial arm, PV was significantly increased at t70 (+138 mL, p = 0.01), t80 (+191 mL, p < 0.01), and t110 (+182 mL, p = 0.01) when compared to t0. Moreover, PV in the ISO trial arm was significantly higher at t70 (p = 0.02), t110 (p = 0.04), and t120 (p = 0.01) when compared to the same time points in the CON trial arm. Within the RIN trial arm, PV was significantly higher between t70 and t90 (+183 mL, p = 0.01) and between t110 (+194 mL, p = 0.03) and t120 (+186 mL, p < 0.01) when compared to t0. These results demonstrated that fluids with a higher content of osmotically active particles lead to acute hemodilution, which is associated with a decrease in [Hb] and Hct. These findings underpin the importance of the hydration state on PV and especially on PV constituent levels in healthy individuals.
摘要：
血浆体积（PV）经历恒定和动态变化，导致健康个体的日内差异很大。众所周知，水合作用会引起PV变化；然而，对渗透不同液体摄入的反应仍未完全理解。在一项随机对照交叉试验中，18名健康个体（10名女性）口服接受了单独量的等渗氯化钠（ISO），振铃(RIN),或葡萄糖（GLU）溶液。用优化的一氧化碳再呼吸方法测定血红蛋白质量(Hbmass)。随后根据毛细血管血红蛋白浓度（[Hb]）和血细胞比容（Hct）计算液体诱导的PV变化，然后每10分钟计算一次，直到液体摄入后120分钟（t0-120），并与对照试验臂（CON）进行比较。没有液体给药。在GLU和CON审判武器内，从基线到t120无统计学差异(p>0.05).在ISO试验臂中，PV在t70时显着增加（+138mL，p=0.01),t80(+191毫升,p<0.01),和t110(+182毫升，当与t0相比时,p=0.01)。此外，ISO试验组的PV在t70时显着较高（p=0.02），t110（p=0.04），和t120(p=0.01)当与CON试验臂中的相同时间点相比时。在RIN试验臂内，PV在t70和t90之间显着升高（+183mL，p=0.01）和t110（+194mL，p=0.03)和t120(+186毫升,当与t0相比时,p<0.01)。这些结果表明，渗透活性颗粒含量较高的液体会导致急性血液稀释，这与[Hb]和Hct的降低有关。这些发现强调了水合状态对PV的重要性，尤其是对健康个体中PV成分水平的重要性。
