PDF(Pubmed)
Abstract：
Talaromyces sp. DC2 is an endophytic fungus that was isolated from the stem of Catharanthus roseus (L.) G. Don in Hanoi, Vietnam and is capable of producing vinca alkaloids. This study utilizes the PacBio Sequel technology to completely sequence the whole genome of Talaromyces sp. DC2The genome study revealed that DC2 contains a total of 34.58 Mb spanned by 156 contigs, with a GC content of 46.5%. The identification and prediction of functional protein-coding genes, tRNA, and rRNA were comprehensively predicted and highly annotated using various BLAST databases, including non-redundant (Nr) protein sequence, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Carbohydrate-Active Enzymes (CAZy) databases. The genome of DC2 has a total of 149, 227, 65, 153, 53, and 6 genes responsible for cellulose, hemicellulose, lignin, pectin, chitin, starch, and inulin degradation, respectively. The Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) analyses revealed that strain DC2 possesses 20 biosynthetic gene clusters responsible for producing secondary metabolites. The strain DC2 has also been found to harbor the DDC gene encoding aromatic L-amino acid decarboxylase enzyme. Conclusively, this study has provided a comprehensive understanding of the processes involved in secondary metabolites and the ability of the Talaromyces sp. DC2 strain to degrade plant cell walls.
摘要：
Talaromycessp.DC2是从长春花茎中分离出的内生真菌（L.)G.唐在河内，越南，能够生产长春花生物碱。这项研究利用PacBioSequel技术对Talaromycessp的整个基因组进行了完全测序。DC2基因组研究显示，DC2总共包含由156个重叠群跨越的34.58Mb，GC含量为46.5%。功能性蛋白质编码基因的鉴定和预测,tRNA,使用各种BLAST数据库对rRNA进行了全面预测和高度注释，包括非冗余（Nr）蛋白质序列，基因本体论（GO），京都基因和基因组百科全书（KEGG），直系同源群（COG），和碳水化合物活性酶(CAZy)数据库。DC2的基因组共有149、227、65、153、53和6个负责纤维素的基因，半纤维素,木质素,果胶,甲壳素,淀粉，和菊粉降解，分别。抗生素和次级代谢物分析壳(AntiSMASH)分析揭示菌株DC2具有负责产生次级代谢物的20个生物合成基因簇。还发现菌株DC2含有编码芳香族L-氨基酸脱羧酶的DDC基因。最后，这项研究提供了对次生代谢产物所涉及的过程和Talaromycessp的能力的全面了解。DC2菌株降解植物细胞壁。
