PDF(Pubmed)
Abstract：
Coccidiomycosis is a potentially life-threatening fungal infection endemic to certain regions of Argentina. The infection is caused by Coccidioides spp. and is primarily diagnosed by Coccidioides antibody (Ab) detection. Access to rapid, highly accurate diagnostic testing is critical to ensure prompt antifungal therapy. The sōna Coccidioides Ab Lateral Flow Assay (LFA) performs faster and requires less laboratory infrastructure and equipment compared with other Ab detection assays, potentially providing a substantial improvement for rapid case screening in coccidioidomycosis-endemic regions; however, validation of this test is needed. Thus, we aimed to evaluate the analytical performance of the sōna Coccidioides Ab (LFA) and compare agreement with anti-Coccidioides Ab detection assays. A total of 103 human sera specimens were tested, including 25 specimens from patients with coccidioidomycosis and 78 from patients without coccidioidomycosis. The sōna Coccidioides Ab Lateral Flow Assay (LFA) was performed with a sensitivity of 88%, and specificity and accuracy of 87%. Furthermore, the Coccidioides Ab LFA had good agreement with other anti-Coccidioides Ab detection assays. Our findings suggest the sōna Coccidioides Ab LFA has satisfactory performance and may be useful for diagnosing coccidioidomycosis in endemic regions.
摘要：
球菌病是阿根廷某些地区特有的潜在威胁生命的真菌感染。感染是由球虫引起的。主要通过球虫抗体（Ab）检测诊断。访问快速，高度准确的诊断测试对于确保及时的抗真菌治疗至关重要。与其他Ab检测测定相比，sōnaCoccidioidesAb侧流测定（LFA）执行速度更快，需要更少的实验室基础设施和设备，可能为球孢子菌病流行地区的快速病例筛查提供实质性改进；然而，需要对此测试进行验证。因此，我们的目的是评估杆纳球虫Ab(LFA)的分析性能,并比较与抗球虫Ab检测试验的一致性.共检测了103份人血清标本,包括25例来自球孢子菌病患者的标本和78例无球孢子菌病患者的标本。以88%的灵敏度进行了球藻抗体侧流测定(LFA),特异性和准确性为87%。此外，球虫AbLFA与其他抗球虫Ab检测试验有良好的一致性.我们的发现表明，s_naCoccidioidesAbLFA具有令人满意的性能，可能有助于在流行地区诊断球虫菌病。
