PDF(Pubmed)
Abstract：
The process of crosslinking improves the physicochemical properties of biopolymer-based composites, making them valuable for biomedical applications. EDC/NHS-crosslinked collagen materials have a significant potential for tissue engineering applications, due to their enhanced properties and biocompatibility. Chemical crosslinking of samples can be carried out in several ways, which is crucial and has a direct effect on the final properties of the obtained material. In this study, the effect of crosslinking conditions on the properties of collagen films using EDC and NHS was investigated. Studies included FTIR spectroscopy, AFM, swelling and degradation tests, mechanical testing and contact angle measurements. Evaluation of prepared collagen films indicated that both crosslinking agents and crosslinking conditions influenced film properties. Notable alternations were observed in the infrared spectrum of the sample, to which EDC was added directly to the fish collagen solution. The same sample indicated the lowest Young modulus, tensile strength and breaking force parameters and the highest elongation at break. All samples reached the maximum swelling degree two hours after immersion in PBS solution; however, the immersion-crosslinked samples exhibited a significantly lower degree of swelling and were highly durable. The highest roughness was observed for the collagen film crosslinked with EDC, whereas the lowest was observed for the specimen crosslinked with EDC with NHS addition. The crosslinking agents increased the surface roughness of the collagen film, except for the sample modified with the addition of EDC and NHS mixture. All films were characterized by hydrophilic character. The films\' modification resulted in a decrease in their hydrophilicity and wettability. Our research allows for a comparison of proposed EDC/NHS crosslinking conditions and their influence on the physicochemical properties of fish collagen thin films. EDC and NHS are promising crosslinking agents for the modification of fish collagen used in biomedical applications.
摘要：
交联过程改善了生物聚合物基复合材料的物理化学性能，使它们对生物医学应用有价值。EDC/NHS交联的胶原蛋白材料在组织工程应用中具有巨大的潜力，由于其增强的性能和生物相容性。样品的化学交联可以通过几种方式进行，这是至关重要的，对所获得材料的最终性能有直接影响。在这项研究中,研究了交联条件对使用EDC和NHS的胶原膜性能的影响。研究包括FTIR光谱，AFM,溶胀和降解试验，机械测试和接触角测量。对制备的胶原膜的评估表明，交联剂和交联条件都会影响膜的性能。在样品的红外光谱中观察到明显的变化，将EDC直接添加到鱼胶原蛋白溶液中。相同的样品显示最低的杨氏模量，拉伸强度和断裂力参数和最高断裂伸长率。所有样品在PBS溶液中浸泡两小时后达到最大溶胀度；然而，浸渍交联的样品表现出明显较低的溶胀度，并且具有很高的耐久性。用EDC交联的胶原膜的粗糙度最高,而与添加NHS的EDC交联的样品观察到最低。交联剂增加了胶原膜的表面粗糙度，除了通过添加EDC和NHS混合物而改性的样品。所有膜的特征在于亲水特性。薄膜改性导致其亲水性和润湿性降低。我们的研究可以比较拟议的EDC/NHS交联条件及其对鱼胶原蛋白薄膜的物理化学性质的影响。EDC和NHS是用于生物医学应用中的鱼胶原蛋白改性的有前途的交联剂。
