PDF(Pubmed)
Abstract：
Periodontal disease is caused by oral pathogenic bacteria and is associated with systemic disease and frailty. Therefore, its prevention is crucial in extending healthy life expectancy. This study aimed to evaluate the effect of orally administered oleanolic acid, extracted from wine pomace, on periodontopathic bacterial growth in healthy individuals. In this randomized, placebo-controlled, double-blind, parallel-group comparison study, 84 healthy adults were assigned to a placebo (n = 29), low-dose (n = 29, 9 mg oleanolic acid), or high-dose (n = 26, 27 mg oleanolic acid) groups. The number of oral bacteria in their saliva, collected before and 5 h after administration, was determined using the polymerase chain reaction-invader technique. The proportion of periodontopathic bacteria among the total oral bacteria in the saliva was calculated. Oleanolic acid significantly decreased the proportion of Porphyromonas gingivalis among the total oral bacteria in a dose-dependent manner (p = 0.005 (low-dose) and p = 0.003 (high-dose) vs. placebo, Williams\' test). Moreover, high-dose oleanolic acid decreased the proportion of Tannerella forsythia (p = 0.064 vs. placebo, Williams\' test). Periodontopathic bacteria are closely associated with the development and progression of periodontal disease; thus, the continuous daily intake of oleanolic acid derived from pomace may be helpful in maintaining a healthy oral microbiome by controlling the proportion of periodontopathic bacteria.
摘要：
牙周病是由口腔致病菌引起的，与全身疾病和虚弱有关。因此,它的预防对于延长健康预期寿命至关重要。本研究旨在评估口服齐墩果酸的效果，从葡萄酒果渣中提取，健康个体的牙周病细菌生长。在这个随机的，安慰剂对照,双盲,平行组比较研究,84名健康成年人被分配给安慰剂(n=29)，低剂量（n=29，9毫克齐墩果酸），或高剂量组（n=26，27mg齐墩果酸）。唾液中口腔细菌的数量，给药前和给药后5小时收集，使用聚合酶链反应-入侵者技术确定。计算唾液中牙周病变细菌在总口腔细菌中的比例。齐墩果酸以剂量依赖性方式显着降低牙龈卟啉单胞菌在总口腔细菌中的比例（p=0.005（低剂量）和p=0.003（高剂量）与安慰剂,威廉姆斯\'测试）。此外，高剂量齐墩果酸可降低连翘坦菌的比例（p=0.064vs.安慰剂,威廉姆斯\'测试）。牙周病菌与牙周病的发生发展密切相关,每天连续摄入来自果渣的齐墩果酸可能有助于通过控制牙周病菌的比例来维持健康的口腔微生物组。
