PDF(Pubmed)
Abstract：
The P1 phage has garnered attention as a carrier of antibiotic resistance genes (ARGs) in Enterobacteriaceae. However, the transferability of ARGs by P1-like phages carrying ARGs, in addition to the mechanism underlying ARG acquisition, remain largely unknown. In this study, we elucidated the biological characteristics, the induction and transmission abilities, and the acquisition mechanism of the blaCTX-M-27 gene in the P1 phage. The P1-CTX phage exhibited distinct lytic plaques and possessed a complete head and tail structure. Additionally, the P1-CTX phage was induced successfully under various conditions, including UV exposure, heat treatment at 42 °C, and subinhibitory concentrations (sub-MICs) of antibiotics. Moreover, the P1-CTX phage could mobilize the blaCTX-M-27 gene into three strains of Escherichia coli (E. coli) and the following seven different serotypes of Salmonella: Rissen, Derby, Kentucky, Typhimurium, Cerro, Senftenberg, and Muenster. The mechanism underlying ARG acquisition by the P1-CTX phage involved Tn1721 transposition-mediated movement of blaCTX-M-27 into the ref and mat genes within its genome. To our knowledge, this is the first report documenting the dynamic processes of ARG acquisition by a phage. Furthermore, this study enriches the research on the mechanism underlying the phage acquisition of drug resistance genes and provides a basis for determining the risk of drug resistance during phage transmission.
摘要：
P1噬菌体作为肠杆菌科中抗生素抗性基因（ARGs）的载体引起了人们的关注。然而,携带ARGs的P1样噬菌体对ARGs的可转移性，除了ARG获取的潜在机制之外，基本上是未知的。在这项研究中,我们阐明了生物学特征，感应和传输能力，和blaCTX-M-27基因在P1噬菌体中的获取机制。P1-CTX噬菌体表现出明显的裂解斑块，并具有完整的头部和尾部结构。此外,在各种条件下成功诱导了P1-CTX噬菌体，包括紫外线照射，42°C热处理,和亚抑制浓度（亚MIC）的抗生素。此外，P1-CTX噬菌体可以将blaCTX-M-27基因动员到三株大肠杆菌中（E.大肠杆菌)和以下七个不同血清型的沙门氏菌：Rissen，德比,肯塔基,鼠伤寒,塞罗,Senftenberg,还有明斯特.P1-CTX噬菌体获得ARG的潜在机制涉及Tn1721转座介导的blaCTX-M-27移入其基因组中的ref和mat基因。据我们所知,这是第一份记录噬菌体获取ARG动态过程的报告。此外，本研究丰富了噬菌体获取耐药基因的机制研究,为噬菌体传播过程中的耐药风险判断提供了依据。
