PDF(Pubmed)
Abstract：
The preparation and processing of rodent brains for evaluation by immunohistochemistry is time-consuming. A large number of mouse brains are routinely used in experiments in neuroscience laboratories to evaluate several models of human diseases. Thus, methods are needed to reduce the time associated with processing brains for histology. A scalable method was developed to embed, section, and stain multiple mouse brains using supplies found in any common histology laboratory. Section collection schemes can be scaled to provide identical bregma locations between adjacent sections for immunohistochemistry, facilitating comprehensive, high-quality immunohistochemistry. As a result, sectioning and staining times are considerably reduced as sections from multiple blocks are stained simultaneously. This method improves on previous procedures and allows multiple embedding and subsequent immunostaining of brains easily with a dramatically reduced time requirement. Furthermore, we expand this method for use in numerous mouse tissues, rat brain tissue, and post-mortem human brain and arterial tissues. In summary, this procedure allows the processing of many rodent or human tissues from perfusion through microscopy in 10 days or less.
摘要：
用于通过免疫组织化学评估的啮齿动物大脑的制备和处理是耗时的。在神经科学实验室的实验中常规使用大量小鼠大脑来评估几种人类疾病模型。因此，需要一些方法来减少与处理大脑进行组织学相关的时间。开发了一种可扩展的方法来嵌入，section,并使用任何常见组织学实验室中的用品对多个小鼠大脑进行染色。可以缩放切片收集方案，以在相邻切片之间提供相同的Bregma位置进行免疫组织化学，促进全面，高质量的免疫组织化学。因此,切片和染色时间大大减少，因为来自多个块的切片同时染色。该方法对先前的程序进行了改进，并且可以轻松地对大脑进行多次嵌入和随后的免疫染色，并大大减少了时间需求。此外，我们将这种方法扩展到许多小鼠组织中，大鼠脑组织,和死后的人脑和动脉组织。总之,该程序允许通过显微镜在10天或更短的时间内从灌注中处理许多啮齿动物或人体组织。
