PDF(Pubmed)
Abstract：
Cystic Echinococcosis (CE) is a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.). This study aims to investigate the use of two monoclonal antibodies (mAbEmG3 and mAbEm2G11) by immunohistochemistry (IHC) to confirm the diagnosis of CE in human patients, in particular in those cases in which other techniques fail to provide a correct or conclusive diagnosis. For this purpose, a survey on 13 patients was performed. These subjects were referred to Sardinian hospitals (Italy) from 2017 to 2022 and were suspected to be affected by CE. Our findings from these 13 patients showed the detection of E. granulosus sensu stricto by IHC in 12 of 13 echinococcal cysts, as one sample was of a non-parasitological origin. The results confirmed that IHC, by means of the mAbEmG3 and mAbEm2G11, is a reliable diagnostic tool that showed a very high performances when tested on strain of E. granulosus s.l. from Sardinia.
摘要：
囊性棘球蚴病（CE）是一种人畜共患疾病，由tape虫细粒棘球蚴（s.l.）的幼虫期引起。这项研究旨在通过免疫组织化学（IHC）研究使用两种单克隆抗体（mAbEmG3和mAbEm2G11）来确认人类患者的CE诊断，特别是在其他技术无法提供正确或决定性诊断的情况下。为此,对13例患者进行了调查。这些受试者从2017年至2022年被转诊到撒丁岛医院（意大利），并被怀疑受到CE的影响。我们从这13例患者的发现显示通过IHC在13个棘球囊囊肿中的12个中检测到严格的细粒E。因为一个样本是非寄生虫学来源的。结果证实IHC,通过mAbEmG3和mAbEm2G11，是一种可靠的诊断工具，当测试来自撒丁岛的E.granulosuss.l.菌株时，显示出非常高的性能。
