PDF(Pubmed)
Abstract：
The memory-enhancing activity of Matricaria chamomilla hydroalcoholic extract (MCE) is already being investigated by behavioral and biochemical assays in scopolamine-induced amnesia rat models, while the effects of scopolamine (Sco) on cerebral glucose metabolism are examined as well. Nevertheless, the study of the metabolic profile determined by an enriched MCE has not been performed before. The present experiments compared metabolic quantification in characteristic cerebral regions and behavioral characteristics for normal, only diseased, diseased, and MCE- vs. Galantamine (Gal)-treated Wistar rats. A memory deficit was induced by four weeks of daily intraperitoneal Sco injection. Starting on the eighth day, the treatment was intraperitoneally administered 30 min after Sco injection for a period of three weeks. The memory assessment comprised three maze tests. Glucose metabolism was quantified after the 18F-FDG PET examination. The right amygdala, piriform, and entorhinal cortex showed the highest differential radiopharmaceutical uptake of the 50 regions analyzed. Rats treated with MCE show metabolic similarity with normal rats, while the Gal-treated group shows features closer to the diseased group. Behavioral assessments evidenced a less anxious status and a better locomotor activity manifested by the MCE-treated group compared to the Gal-treated group. These findings prove evident metabolic ameliorative qualities of MCE over Gal classic treatment, suggesting that the extract could be a potent neuropharmacological agent against amnesia.
摘要：
在东pol碱诱导的健忘症大鼠模型中，已经通过行为和生化试验研究了母草水醇提取物（MCE）的记忆增强活性，同时研究了东莨菪碱（Sco）对脑葡萄糖代谢的影响。然而，之前尚未对富集MCE确定的代谢谱进行研究。本实验比较了正常大脑特征区域和行为特征的代谢定量，只有患病，患病,和MCE-vs.加兰他敏(Gal)处理的Wistar大鼠。每天腹膜内注射Sco可引起记忆缺陷。从第八天开始，治疗在Sco注射后30分钟腹膜内给药，为期3周。记忆评估包括三个迷宫测试。在18F-FDGPET检查后定量葡萄糖代谢。右边的杏仁核,梨形,在分析的50个区域中，内嗅皮层显示出最高的差异放射性药物吸收。用MCE处理的大鼠显示与正常大鼠的代谢相似性,而Gal治疗组显示出更接近患病组的特征。行为评估证明,与Gal治疗组相比,MCE治疗组表现出更少的焦虑状态和更好的运动活动。这些发现证明了MCE相对于Gal经典治疗的明显代谢改善特性，这表明该提取物可能是一种有效的抗健忘症神经药物。
