Abstract：
In this study, we investigated the prevalence of Bartonella in deer from Qilian County, Qinghai Province, China. Blood samples were collected from 69 red deer, 40 white-lipped deer, and 27 sika deer. The detection of Bartonella spp. has been conducted. The overall prevalence of Bartonella was 33.6% (46/135). Species-specific prevalence was 50.72% in red deer (35/69), 20.00% in white-lipped deer (8/40), and 11.11% in sika deer (3/27). There were significant differences in the prevalence rates among the different species of deer. The amplicon sequence comparison revealed a high homology of the ruminant-associated Bartonella spp. Nanopore sequencing further confirmed the results. Bartonella reads were presented in each of the qPCR-positive samples. Phylogenetic analysis indicated that the Bartonella sequences detected in deer blood were closely related to ruminant-borne Bartonella spp. In summary, we reported the Bartonella prevalence of different deer species in Qinghai, and there were at least one species of ruminant-associated Bartonella, B. schoenbuchensis.
OBJECTIVE: This is the first report about Bartonella infections in the deer population from China. We found that there were two species of Bartonella and an unidentified species of Bartonella among the unculturing strains carried by these deer populations. We first used Nanopore sequencing to detect Bartonella from deer blood samples and indicated that Nanopore sequencing is beneficial to detect pathogens due to its advantage of real-time and high sensitivity.
OBJECTIVE: This is the first report about Bartonella infections in the deer population from China. We found that there were two species of Bartonella and an unidentified species of Bartonella among the unculturing strains carried by these deer populations. We first used Nanopore sequencing to detect Bartonella from deer blood samples and indicated that Nanopore sequencing is beneficial to detect pathogens due to its advantage of real-time and high sensitivity.
摘要：
在这项研究中,我们调查了祁连县鹿中巴尔通体的流行情况，青海省,中国。采集了69只马鹿的血样,40只白唇鹿,和27只梅花鹿。巴尔通体的检测。已经进行了。巴尔通体的总体患病率为33.6％（46/135）。马鹿的物种特异性患病率为50.72%(35/69)，白唇鹿20.00%（8/40），梅花鹿为11.11%(3/27)。不同种类鹿的患病率存在显着差异。扩增子序列比较揭示了与反刍动物相关的巴尔通体物种的高度同源性。纳米孔测序进一步证实了该结果。在每个qPCR阳性样品中呈现巴尔通体读数。系统发育分析表明，在鹿血中检测到的巴尔通体序列与反刍动物传播的巴尔通体密切相关。总之,我们报道了青海不同种类鹿的巴尔通体流行情况，至少有一种与反刍动物相关的巴尔通体，B.schoenbuchensis。
目的：这是关于中国鹿群巴尔通体感染的首次报道。我们发现，在这些鹿种群携带的未培养菌株中，有两种巴尔通体和一种身份不明的巴尔通体。我们首次使用Nanopore测序技术检测鹿血液样本中的Bartonella，并表明Nanopore测序技术由于其实时和高灵敏度的优势，有利于检测病原体。
目的：这是关于中国鹿群巴尔通体感染的首次报道。我们发现，在这些鹿种群携带的未培养菌株中，有两种巴尔通体和一种身份不明的巴尔通体。我们首次使用Nanopore测序技术检测鹿血液样本中的Bartonella，并表明Nanopore测序技术由于其实时和高灵敏度的优势，有利于检测病原体。
