Abstract：
UNASSIGNED: Asiaticoside (AS) has been reported to improve the changes induced by high glucose stimulation, and it may have potential therapeutic effects on gestational diabetes mellitus (GDM). This study aims to explore the effect of AS on the cell model of GDM and the action mechanism of the PI3K/AKT pathway.
UNASSIGNED: The GDM model was established in HTR-8/Svneo cells with a high glucose (HG) medium. After the cytotoxicity assay of AS, cells were divided into the control group, HG group and HG + AS group to conduct control experiment in cells. The cell proliferation and migration were detected by CCK-8 assay and scratch test, respectively. The mRNA levels of PI3K, AKT2, mTORC1, and GLUT4 in PI3K/AKT signalling pathway were measured by RT-PCR, and the protein expressions of these signalling molecules were monitored by western blot.
UNASSIGNED: AS showed a promotion effect on the cell proliferation rate of HTR-8/Svneo cells, and 80 μmol/L AS with a treatment time of 48 h had no cytotoxicity. The cell proliferation rate, migration rate, mRNA levels and protein expressions of PI3K, AKT2, mTORC1, and GLUT4 in the HG group were significantly lower than those in the control group, which were significantly increased in the HG + AS group (p < 0.05).
UNASSIGNED: AS can facilitate the cell proliferation and migration in the cell model of GDM, and might play a role in GDM treatment via PI3K/AKT pathway.
Asiaticoside possesses various pharmacological effects and has been reported to show a beneficial effect on the treatment of diabetes mellitus. This research firstly investigated the effect and mechanism of asiaticoside on gestational diabetes mellitus, and found that asiaticoside could facilitate the cell proliferation and migration of HTR-8/Svneo cells treated with high glucose, and affect the signalling molecules of PI3K/AKT pathway. Therefore, asiaticoside may be a novel useful therapeutic drug in the treatment of gestational diabetes mellitus.
UNASSIGNED: The GDM model was established in HTR-8/Svneo cells with a high glucose (HG) medium. After the cytotoxicity assay of AS, cells were divided into the control group, HG group and HG + AS group to conduct control experiment in cells. The cell proliferation and migration were detected by CCK-8 assay and scratch test, respectively. The mRNA levels of PI3K, AKT2, mTORC1, and GLUT4 in PI3K/AKT signalling pathway were measured by RT-PCR, and the protein expressions of these signalling molecules were monitored by western blot.
UNASSIGNED: AS showed a promotion effect on the cell proliferation rate of HTR-8/Svneo cells, and 80 μmol/L AS with a treatment time of 48 h had no cytotoxicity. The cell proliferation rate, migration rate, mRNA levels and protein expressions of PI3K, AKT2, mTORC1, and GLUT4 in the HG group were significantly lower than those in the control group, which were significantly increased in the HG + AS group (p < 0.05).
UNASSIGNED: AS can facilitate the cell proliferation and migration in the cell model of GDM, and might play a role in GDM treatment via PI3K/AKT pathway.
Asiaticoside possesses various pharmacological effects and has been reported to show a beneficial effect on the treatment of diabetes mellitus. This research firstly investigated the effect and mechanism of asiaticoside on gestational diabetes mellitus, and found that asiaticoside could facilitate the cell proliferation and migration of HTR-8/Svneo cells treated with high glucose, and affect the signalling molecules of PI3K/AKT pathway. Therefore, asiaticoside may be a novel useful therapeutic drug in the treatment of gestational diabetes mellitus.
摘要：
■据报道，积雪草苷（AS）可以改善高糖刺激引起的变化，它可能对妊娠期糖尿病（GDM）具有潜在的治疗作用。本研讨旨在摸索AS对GDM细胞模子的影响及PI3K/AKT通路的感化机制。
■在具有高葡萄糖（HG）培养基的HTR-8/Svneo细胞中建立GDM模型。在AS的细胞毒性测定后，细胞分为对照组，HG组和HG+AS组进行细胞对照实验。CCK-8法和划痕试验检测细胞增殖和迁移,分别。PI3K的mRNA水平,通过RT-PCR检测PI3K/AKT信号通路中的AKT2、mTORC1和GLUT4,并通过蛋白质印迹监测这些信号分子的蛋白表达。
■AS对HTR-8/Svneo细胞的细胞增殖率有促进作用，80μmol/LAS处理时间48h无细胞毒性。细胞增殖率,迁移率,PI3K的mRNA水平和蛋白表达,HG组AKT2、mTORC1、GLUT4明显低于对照组,HG+AS组明显升高(p<0.05)。
■AS可以促进GDM细胞模型中的细胞增殖和迁移，并可能通过PI3K/AKT途径在GDM治疗中发挥作用。
积雪草苷具有多种药理作用,据报道显示出治疗糖尿病的有益效果。本研究首先探讨积雪草苷对妊娠期糖尿病的作用及机制,并发现积雪草苷可以促进高糖处理的HTR-8/Svneo细胞的增殖和迁移，并影响PI3K/AKT通路的信号分子。因此,积雪草苷可能是治疗妊娠期糖尿病的一种新型药物。
■在具有高葡萄糖（HG）培养基的HTR-8/Svneo细胞中建立GDM模型。在AS的细胞毒性测定后，细胞分为对照组，HG组和HG+AS组进行细胞对照实验。CCK-8法和划痕试验检测细胞增殖和迁移,分别。PI3K的mRNA水平,通过RT-PCR检测PI3K/AKT信号通路中的AKT2、mTORC1和GLUT4,并通过蛋白质印迹监测这些信号分子的蛋白表达。
■AS对HTR-8/Svneo细胞的细胞增殖率有促进作用，80μmol/LAS处理时间48h无细胞毒性。细胞增殖率,迁移率,PI3K的mRNA水平和蛋白表达,HG组AKT2、mTORC1、GLUT4明显低于对照组,HG+AS组明显升高(p<0.05)。
■AS可以促进GDM细胞模型中的细胞增殖和迁移，并可能通过PI3K/AKT途径在GDM治疗中发挥作用。
积雪草苷具有多种药理作用,据报道显示出治疗糖尿病的有益效果。本研究首先探讨积雪草苷对妊娠期糖尿病的作用及机制,并发现积雪草苷可以促进高糖处理的HTR-8/Svneo细胞的增殖和迁移，并影响PI3K/AKT通路的信号分子。因此,积雪草苷可能是治疗妊娠期糖尿病的一种新型药物。
