PDF(Pubmed)
Abstract：
DNA methylation is one of induced changes under salinity stress causing reduction in the expression of several crucial genes required for normal plant\'s operation. Potential use of royal jelly (RJ), folic acid (FA) and 5-azacitidine (5-AZA) on two Egyptian faba bean varieties (Sakha-3 and Giza-716) grown under saline conditions was investigated. Salinity stress affects negatively on seeds germination (G %), mitotic index, membrane stability and induced a significant increase in chromosomal abnormalities (CAs). DNA methyltransferases genes (MT1 and MT2) were highly up-regulated (∼23 and 8 folds for MT1 and MT2 in shoots of Giza-716 stressed plants). On the other hand, down regulation of other studied stress related genes: superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), heat shock protein (HSP-17.9) and proline-rich protein (GPRP) were detected in stressed plants of both studied varieties. Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants. Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2 which was associated with significant up regulation in the expression of Cu/Zn-SOD, CAT, GR, GPRP and HSP-17.9 encoding genes. The Lowest expression of MT1 and MT2 were induced with 5-AZA treatment in both studied varieties. Exogenous application of the FA, RJ and 5-AZA modified the methylation state of stressed plants by regulation the expression of DNA methyltransferases, subsequently, modulated the expression of studied genes and could be proposed as a promising treatment to ameliorate hazardous effects of salt stress on different plants.
摘要：
DNA甲基化是在盐度胁迫下引起植物正常运行所需的几个关键基因表达减少的诱导变化之一。蜂王浆（RJ）的潜在用途，研究了在盐水条件下生长的两个埃及蚕豆品种（Sakha-3和Giza-716）的叶酸（FA）和5-阿扎胞苷（5-AZA）。盐度胁迫对种子萌发有负面影响（G%），有丝分裂指数,膜稳定性和诱导染色体异常（CA）的显着增加。DNA甲基转移酶基因（MT1和MT2）高度上调（在Giza-716胁迫植物的芽中，MT1和MT2的〜23和8倍）。另一方面,其他研究的胁迫相关基因的下调：超氧化物歧化酶（SOD），过氧化氢酶(CAT),谷胱甘肽还原酶（GR），在两个研究品种的胁迫植物中检测到热休克蛋白（HSP-17.9）和富含脯氨酸的蛋白（GPRP）。用RJ和FA处理植物增加G%，叶绿素含量，与未处理的胁迫植物相比，提高了膜性能并减少了CA。5-AZA的外源性应用，RJ和FA对盐度胁迫的植物与MT1和MT2转录的显着降低有关，这与Cu/Zn-SOD表达的显着上调有关，CAT,GR,GPRP和HSP-17.9编码基因。在两个研究品种中，用5-AZA处理诱导了最低的MT1和MT2表达。FA的外源性应用，RJ和5-AZA通过调节DNA甲基转移酶的表达来修饰受胁迫植物的甲基化状态，随后,调节所研究基因的表达，可作为改善盐胁迫对不同植物的有害影响的有希望的处理方法。
