METHODS: We extracted the outer layers of 30 human teeth to expose the tubules and then treated the surfaces with 17% ethylenediaminetetraacetic acid (EDTA) to create an etched effect. Three cohorts were created from the portions. Group A was subjected to the application of Erbium:Yttrium-Aluminum-Garnet (Er: YAG) laser with a power output of 2W in the non-contact mode for 1 minute. Group B was subjected to the application of an 810nm diode laser with a power output of 1W in continuous mode for 30 seconds. Group C was subjected to the application of NovaMin paste, which contains a 927 ppm fluoride content. Following the therapy, occluded dentinal tubules were analyzed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) for both quantitative and qualitative examination. The data analysis was conducted using a one-way analysis of variance (ANOVA) and Tukey\'s test, with a significance threshold of 0.05.
RESULTS: The average percentages of complete blockage of dentinal tubules in Groups A, B, and C were evaluated using the number of entirely unobstructed dentinal tubules at magnifications of 2000X (F = 3.05, p = 0.064), 5000X (F = 5.33, p = 0.011), and 10000X (F = 8.63, p = 0.001). The count of partially open dentinal tubules seen at magnifications of 2000X, 5000X, and 10000X was F = 10.15 (P < 0.001), F = 5.97 (p = 0.007), and F = 2.12 (p = 0.140) accordingly.
CONCLUSIONS: NovaMin technology has demonstrated more effectiveness in blocking dentinal tubules compared to 810nm diodes and Er: YAG lasers.
方法:我们提取了30颗人类牙齿的外层以暴露小管,然后用17%的乙二胺四乙酸(EDTA)处理表面以产生蚀刻效果。从这些部分创建了三个队列。A组在非接触模式下施加功率为2W的铒:钇-铝-石榴石(Er:YAG)激光器1分钟。B组在连续模式下施加功率输出为1W的810nm二极管激光器30秒。C组接受NovaMin糊剂的应用,其中含有927ppm的氟化物含量。治疗后,使用扫描电子显微镜(SEM)和能量色散X射线光谱(EDX)对闭塞的牙本质小管进行定量和定性检查。数据分析采用单因素方差分析(ANOVA)和Tukey检验,显著性阈值为0.05。
结果:A组牙本质小管完全阻塞的平均百分比,B,和C使用2000倍放大倍数的完全通畅的牙本质小管的数量进行评估(F=3.05,p=0.064),5000X(F=5.33,p=0.011),和10000X(F=8.63,p=0.001)。放大2000倍观察到的部分开放的牙本质小管的计数,5000X,10000X为F=10.15(P<0.001),F=5.97(p=0.007),F=2.12(p=0.140)。
结论:与810nm二极管和Er:YAG激光器相比,NovaMin技术在阻断牙本质小管方面表现出更高的有效性。