Abstract：
The insulin signaling (IIS) pathway plays a key role in the regulation of various physiological functions in animals. However, the involvement of IIS pathway in the reproduction of natural enemy insects remains enigmatic. Here, 3 key genes (named ClInR, ClPI3K, and ClAKT) related to IIS pathway were cloned from Cyrtorhinus lividipennis (Reuter) (Hemiptera: Miridae), an important natural enemy in the rice ecosystem. These 3 proteins had the typical features of corresponding protein families and shared high similarity with their respective homologs from the Hemipteran species. The ClInR, ClPI3K, and ClAKT were highly expressed in the adult stage. Tissue distribution analysis revealed that ClInR, ClPI3K, and ClAKT were highly expressed in the midgut and ovary of adults. Silencing of ClInR, ClPI3K, and ClAKT caused 92.1%, 72.1%, and 57.8% reduction in the expression of ClVg, respectively. Depletion of these 3 genes impaired vitellogenin synthesis and ovary development. Moreover, the fecundity in the dsInR, dsPI3K, and dsAKT injected females were 53.9%, 50.8%, and 48.5% lower than the control treatment, respectively. These results indicated that ClInR, ClPI3K, and ClAKT are of great importance for the reproduction of C. lividipennis. Our results advance the knowledge about the molecular mechanism of reproduction regulation in natural enemy insects.
摘要：
胰岛素信号（IIS）通路在动物各种生理功能的调节中起着关键作用。然而,IIS途径参与天敌昆虫的繁殖仍然是一个谜。这里,3个关键基因（命名为ClInR，ClPI3K,和ClAKT）与IIS途径相关的克隆来自白鲸（Reuter）（半翅目：Miridae），水稻生态系统中的重要天敌。这3种蛋白质具有相应蛋白质家族的典型特征，并且与半翅目物种的各自同源物具有高度相似性。ClInR,ClPI3K,ClAKT在成年期高表达。组织分布分析显示，ClInR，ClPI3K,ClAKT在成人中肠和卵巢中高表达。ClInR的沉默,ClPI3K,和ClAKT造成92.1％，72.1%,ClVg的表达减少了57.8%，分别。这3个基因的缺失会损害卵黄蛋白原的合成和卵巢发育。此外，dsInR中的繁殖力，dsPI3K,注射dsAKT的女性占53.9%，50.8%,比对照治疗低48.5%，分别。这些结果表明，ClInR，ClPI3K,和ClAKT对于C.lividipennis的繁殖非常重要。我们的结果增进了有关天敌昆虫繁殖调节的分子机制的知识。
