Abstract：
The lobed leaves of rapeseed (Brassica napus L.) offer significant advantages in dense planting, leading to increased yield. Although AtWIP2, a C2H2 zinc finger transcription factor, acts as a regulator of leaf development in Arabidopsis thaliana, the function and regulatory mechanisms of BnaWIP2 in B. napus remain unclear. Here, constitutive expression of the BnaC06.WIP2 paralog, predominantly expressed in leaf serrations, produced lobed leaves in both A. thaliana and B. napus. We demonstrated that BnaC06.WIP2 directly repressed the expression of BnaA01.TCP4, BnaA03.TCP4, and BnaC03.TCP4 and indirectly inhibited the expression of BnaA05.BOP1 and BnaC02.AS2 to promote leaf lobe formation. On the other hand, we discovered that BnaC06.WIP2 modulated the levels of endogenous gibberellin, cytokinin, and auxin, and controlled the auxin distribution in B. napus leaves, thus accelerating leaf lobe formation. Meanwhile, we revealed that BnaA09.STM physically interacted with BnaC06.WIP2, and ectopic expression of BnaA09.STM generated smaller and lobed leaves in B. napus. Furthermore, we found that BnaC06.WIP2 and BnaA09.STM synergistically promoted leaf lobe formation through forming transcriptional regulatory module. Collectively, our findings not only facilitate in-depth understanding of the regulatory mechanisms underlying lobed leaf formation, but also are helpful for guiding high-density breeding practices through improving leaf morphology in B. napus.
摘要：
油菜籽（BrassicanapusL.）的浅叶在密集种植中具有显着的优势，导致产量增加。虽然C2H2锌指转录因子AtWIP2，作为拟南芥叶片发育的调节剂，BnaWIP2在甘蓝型油菜中的功能和调控机制尚不清楚。这里,BnaC06的组成型表达。WIP2模拟，主要在叶锯齿中表达，在拟南芥和甘蓝型油菜中都产生了叶状叶子。我们证明了BnaC06。WIP2直接抑制BnaA01的表达。TCP4,BnaA03.TCP4和BnaC03。TCP4并间接抑制BnaA05的表达。BOP1和BnaC02。AS2促进叶叶形成。另一方面,我们发现了BnaC06.WIP2调节内源性赤霉素的水平，细胞分裂素,和生长素,并控制了油菜叶片中生长素的分布，从而加速叶叶的形成。同时,我们发现BnaA09.STM与BnaC06物理相互作用。WIP2和BnaA09的异位表达。STM在甘蓝型油菜中产生较小的浅叶。此外，我们发现了BnaC06.WIP2和BnaA09。STM通过形成转录调控模块协同促进叶叶形成。总的来说，我们的发现不仅有助于深入了解叶状叶形成的调节机制，而且还有助于通过改善甘蓝型油菜叶片形态来指导高密度育种实践。
