Abstract：
General anesthesia and surgical stress can suppress the immunological response by acting both directly on the immune system and indirectly on the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system. Disturbance of the immune system during the perioperative period can lead to complications such as wound-healing disorders and infections up to sepsis. Effectiveness of acupuncture in regulating the immune function by increasing leukocyte numbers and inhibiting inflammatory response has been proven. This study aimed to explore the impact of electroacupuncture (EAP) on the dynamic balance of the immune system and immune cell populations in dogs undergoing surgery. Twelve healthy bitches scheduled for elective ovariectomy were divided into two groups according to whether (EAP, n=6) or not (CTR, n=6) a peri-operative electroacupuncture treatment was performed. Levels of leukocytes (neutrophils, monocytes, T- and B-cells) and immunoglobulins M (IgM) and A (IgA) were measured in blood samples collected before (T0), 1 h (T1) and 2.5 h (T2) after anesthesia induction. Leukocytes count decreased from T0 to T1 in both groups and restored within 1.5 h in EAP group whereas remained significantly lower in CTR group (P<0.02). In particular, neutrophils and monocytes increased in dogs receiving EAP (P<0.01) while T-cells decreased in CTR group (P<0.04) at T2. B-cells and cytotoxic T-cells decreased in EAP dogs (P<0.04) at T2. No differences in helper T-cells, IgM and IgA levels were recorded between groups and over time. Our results suggest a modulatory effect of EAP on the immune system which is early expressed on neutrophils, monocytes and T-cells.
摘要：
全身麻醉和手术应激可以通过直接作用于免疫系统和间接作用于下丘脑-垂体-肾上腺轴和交感神经系统来抑制免疫反应。围手术期免疫系统的紊乱可导致并发症，例如伤口愈合障碍和感染直至败血症。针刺通过增加白细胞数量和抑制炎症反应来调节免疫功能的有效性已被证明。本研究旨在探讨电针(EAP)对手术犬免疫系统和免疫细胞群动态平衡的影响。根据是否（EAP，n=6)或不(CTR，n=6）进行围手术期电针治疗。白细胞水平（中性粒细胞，单核细胞,T细胞和B细胞）以及免疫球蛋白M（IgM）和A（IgA）在之前（T0）收集的血液样本中进行测量，麻醉诱导后1小时(T1)和2.5小时(T2)。从T0到T1，两组白细胞计数均下降，EAP组在1.5小时内恢复，而CTR组则显着降低（P<0.02）。特别是,在T2时，接受EAP的犬中性粒细胞和单核细胞增加（P<0.01），而CTR组T细胞减少（P<0.04）。在T2时，EAP犬的B细胞和细胞毒性T细胞减少（P<0.04）。辅助性T细胞没有差异，在组间和随时间记录IgM和IgA水平。我们的结果表明，EAP对免疫系统的调节作用，早期在中性粒细胞上表达，单核细胞和T细胞。
