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Abstract：
Posterior Capsule Opacification (PCO), the most frequent complication of cataract surgery, is caused by the infiltration and proliferation of lens epithelial cells (LECs) at the interface between the intraocular lens (IOL) and posterior lens capsule (PLC). According to the \"no space, no cells, no PCO\" theory, high affinity (or adhesion force) between the IOL and PLC would decrease the IOL: PLC interface space, hinder LEC migration, and thus reduce PCO formation. To test this hypothesis, an in vitro hemisphere-shaped simulated PLC (sPLC) was made to mimic the human IOL: PLC physical interactions and to assess their influence on LEC responses. Three commercially available IOLs with different affinities/adhesion forces toward the sPLC, including Acrylic foldable IOL, Silicone IOL, and PMMA IOL, were used in this investigation. Using the system, the physical interactions between IOLs and sPLC were quantified by measuring the adhesion force and interface space using an adhesion force apparatus and Optical Coherence Tomography, respectively. Our data shows that high adhesion force and tight binding between IOL and sPLC contribute to a small interface space (or \"no space\"). By introducing LECs into the in vitro system, we found that, with small interface space, among all IOLs, acrylic foldable IOLs permitted the least extent of LEC infiltration, proliferation, and differentiation (or \"no cells\"). Further statistical analyses using clinical data revealed that weak LEC responses are associated with low clinical PCO incidence rates (or \"no PCO\"). The findings support that the in vitro system could simulate IOL: PLC interplays and predict IOLs\' PCO potential in support of the \"no space, no cells, no PCO\" hypothesis.
摘要：
后囊混浊(PCO),白内障手术最常见的并发症，是由人工晶状体（IOL）和晶状体后囊（PLC）之间的界面处的晶状体上皮细胞（LECs）的浸润和增殖引起的。根据“没有空间”，没有细胞,没有PCO理论，IOL和PLC之间的高亲和力（或粘附力）会减少IOL：PLC接口空间，阻碍LEC迁移，从而减少PCO的形成。为了检验这个假设，体外半球形模拟PLC(sPLC)模拟人IOL:PLC物理相互作用,并评估其对LEC反应的影响.三种市售IOL对sPLC具有不同的亲和力/附着力，包括丙烯酸可折叠IOL，硅胶IOL,和PMMAIOL，在这次调查中使用。使用该系统，通过使用粘合力装置和光学相干断层扫描测量粘合力和界面空间来量化IOL和sPLC之间的物理相互作用，分别。我们的数据表明，IOL和sPLC之间的高粘附力和紧密结合有助于小的界面空间（或“无空间”）。通过将LEC引入体外系统，我们发现,接口空间小，在所有IOL中，丙烯酸可折叠IOL允许最小程度的LEC渗透，扩散,和分化（或“无细胞”）。使用临床数据的进一步统计分析显示，弱LEC反应与低临床PCO发生率（或“无PCO”）相关。研究结果支持体外系统可以模拟IOL：PLC相互作用并预测IOL的PCO潜力，以支持“无空间”，没有细胞,没有PCO假设。
