Abstract：
CONCLUSIONS: TaAGL66, a MADS-box transcription factor highly expressed in fertile anthers of KTM3315A, regulates anther and/or pollen development, as well as male fertility in wheat with Aegilops kotschyi cytoplasm. Male sterility, as a string of sophisticated biological processes in higher plants, is commonly regulated by transcription factors (TFs). Among them, MADS-box TFs are mainly participated in the processes of floral organ formation and pollen development, which are tightly related to male sterility, but they have been little studied in the reproductive development in wheat. In our study, TaAGL66, a gene that was specifically expressed in spikes and highly expressed in fertile anthers, was identified by RNA sequencing and the expression profiles data of these genes, and qRT-PCR analyses, which was localized to the nucleus. Silencing of TaAGL66 under fertility condition in KTM3315A, a thermo-sensitive male sterile line with Ae. kotschyi cytoplasm, displayed severe fertility reduction, abnormal anther dehiscence, defective pollen development, decreased viability, and low seed-setting. It can be concluded that TaAGL66 plays an important role in wheat pollen development in the presence of Ae. kotschyi cytoplasm, providing new insights into the utilization of male sterility.
摘要：
结论：TaAGL66，一种在KTM3315A可育花药中高表达的MADS-box转录因子，调节花药和/或花粉发育，以及具有Aegilopskotschyi细胞质的小麦的雄性生育力。男性不育,作为高等植物中一系列复杂的生物过程，通常由转录因子(TF)调节。其中,MADS-boxTFs主要参与花器官形成和花粉发育过程,这与男性不育密切相关，但是它们在小麦生殖发育中的研究很少。在我们的研究中,TaAGL66是一种在穗中特异性表达并在可育花药中高度表达的基因，通过RNA测序和这些基因的表达谱数据鉴定，和qRT-PCR分析，位于细胞核。KTM3315A育性条件下TaAGL66的沉默，具有Ae的热敏雄性不育系。kotschyi细胞质，显示出严重的生育率下降，异常的花药开裂，花粉发育缺陷，生存能力下降，和低种子。可以得出结论，在Ae的存在下，TaAGL66在小麦花粉发育中起着重要的作用。kotschyi细胞质，为雄性不育的利用提供新的见解。
