Abstract：
We aimed to evaluate the effects of the antioxidant thymoquinone on treated and untreated kidneys on histological and oxidative parameters as well as Kidney Injury Molecule (KIM-1) levels in an experimental unilateral ureteropelvic junction obstruction (UPJO) with resultant hydronephrosis (HN) model. In adherence to the Animal research: reporting of in vivo exepriments guidelines, 34 male Wistar rats were randomly divided into four groups which were named accordingly: \"CO\" (corn oil), \"TQ\" (thymoquinone and corn oil), \"HNCO\" (UPJO-HN and corn oil), \"HNTQ\" (UPJO-HN, thymoquinone and corn oil). Histologically, pelvic epithelial damage, glomerular shrinkage and sclerosis, tubular damage, interstitial edema-inflammation-fibrosis (IEIF), and vascular congestion were assessed. Biochemically, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione reductase (GR) and KIM-1 levels were assessed. Macroscopic HN developed in all obstructed kidneys. Ipsilateral obstructed kidneys deteriorated in all histological parameters. Thymoquinone attenuated glomerular shrinkage and sclerosis alterations but increased vascular congestion. Contralateral non-obstructed kidneys also showed histological deterioration. Thymoquinone had beneficial effects in terms of IEIF presence in contralateral kidneys but it increased vascular congestion. MDA and SOD results were inconclusive. UPJO caused decreased GR levels in the ipsilateral kidneys but not in the contralateral ones. This effect was not ameliorated by thymoquinone treatment. KIM-1 levels were increased in ipsilateral obstructed kidneys with a lower level in HNTQ group than in HNCO. KIM-1 level of the ipsilateral HNTQ group was higher than in both non-obstructed ipsilateral kidney groups. The effect of thymoquinone in ameliorating bilaterally observed histological alterations was limited and controversial. Oxidative damage detected by GR measurements was not prevented by thymoquinone. Thymoquinone partially decreased the damage as evidenced by reduced KIM-1 levels in thymoquinone-treated obstructed kidneys.
摘要：
我们旨在评估抗氧化剂百里香醌对实验性单侧肾盂输尿管连接梗阻（UPJO）伴肾积水（HN）模型中治疗和未治疗肾脏的组织学和氧化参数以及肾损伤分子（KIM-1）水平的影响。遵守动物研究：体内实验指南的报告，将34只雄性Wistar大鼠随机分为四组，分别命名为：“CO”（玉米油），“TQ”（百里香醌和玉米油），“HNCO”（UPJO-HN和玉米油），\"HNTQ\"(UPJO-HN,百里香醌和玉米油）。组织学上,盆腔上皮损伤,肾小球收缩和硬化，管道损坏,间质水肿-炎症-纤维化（IEIF），和血管充血进行评估。生物化学,丙二醛（MDA），超氧化物歧化酶（SOD），评估谷胱甘肽还原酶（GR）和KIM-1水平。所有阻塞肾脏均出现宏观HN。同侧阻塞肾脏的所有组织学参数均恶化。胸腺醌减弱了肾小球收缩和硬化改变，但增加了血管充血。对侧未阻塞的肾脏也显示出组织学恶化。胸醌在对侧肾脏中存在IEIF方面具有有益作用，但增加了血管充血。MDA和SOD结果尚无定论。UPJO导致同侧肾脏的GR水平降低，而对侧肾脏则没有。百里香醌治疗没有改善这种作用。同侧梗阻肾脏的KIM-1水平升高，HNTQ组的水平低于HNCO。同侧HNTQ组的KIM-1水平高于两个未阻塞的同侧肾组。百里香醌改善双侧观察到的组织学改变的作用是有限且有争议的。通过GR测量检测到的氧化损伤没有被百里香醌阻止。百里香醌部分减少了损害，如百里香醌治疗的阻塞肾脏中KIM-1水平降低所证明。
