METHODS: The serum samples from patients with NASH (female, n = 9) and SS (female, n = 4; male, n = 2) were used for isolation of LDL. Total lipids were extracted from isolated LDL, and the species of PC and PC-OOH were measured using liquid chromatography-mass spectrometry/mass spectrometry.
RESULTS: The sum of LDL-PC and the sum of LDL-PCOOH were significantly higher in NASH than in SS. Several LDL-PC (PC 32:0, 32:1, 32:2, 34:3, 36:2, sum of PC with saturated fatty acyl chains and sum of LDL-PC with polyunsaturated fatty acyl chains) and several LDL-PCOOH (34:2, 36:2, 36:3 and total) were increased significantly with increasing fibrosis score. In particular, a series of LDL-PCOOH were more reflective of the severity of fibrosis score.
CONCLUSIONS: LDL-PC and LDL-PCOOH species were strongly correlated with the fibrosis score in NASH, which suggests that abnormal LDL is involved in the development of liver fibrosis.
方法:NASH患者的血清样本(女性,n=9)和SS(女性,n=4;男性,n=2)用于分离LDL。从分离的LDL中提取总脂质,使用液相色谱-质谱/质谱法测量PC和PC-OOH的种类。
结果:NASH中LDL-PC和LDL-PCOOH的总和明显高于SS。几个LDL-PC(PC32:0,32:1,32:2,34:3,36:2,具有饱和脂肪酰基链的PC总和和具有多不饱和脂肪酰基链的LDL-PC总和)和几个LDL-PCOOH(34:2,36:2,36:3和总计)随着纤维化评分的增加而显着增加。特别是,一系列LDL-PCOOH更能反映纤维化评分的严重程度.
结论:LDL-PC和LDL-PCOOH物种与NASH的纤维化评分密切相关,这表明异常LDL参与肝纤维化的发展。