Abstract：
BACKGROUND: Non-alcoholic fatty liver disease is classified into simple steatosis (SS) and non-alcoholic steatohepatitis (NASH) according to histological findings from liver biopsies. Phosphatidylcholine (PC), the main component of phospholipids in serum lipoproteins, is easily oxidized to phosphatidylcholine hydroperoxide (PC-OOH). Although a lipid composition in the low-density lipoproteins (LDL) from patients with NASH could be abnormal, it remains unclear. Here, to better understand the characteristics of lipids in the LDL from NASH and SS, we compared the composition of PC and PC-OOH species in LDL particles (LDL-PC, LDL-PCOOH) from these patients, then clarified the association between these lipids and NASH severity.
METHODS: The serum samples from patients with NASH (female, n = 9) and SS (female, n = 4; male, n = 2) were used for isolation of LDL. Total lipids were extracted from isolated LDL, and the species of PC and PC-OOH were measured using liquid chromatography-mass spectrometry/mass spectrometry.
RESULTS: The sum of LDL-PC and the sum of LDL-PCOOH were significantly higher in NASH than in SS. Several LDL-PC (PC 32:0, 32:1, 32:2, 34:3, 36:2, sum of PC with saturated fatty acyl chains and sum of LDL-PC with polyunsaturated fatty acyl chains) and several LDL-PCOOH (34:2, 36:2, 36:3 and total) were increased significantly with increasing fibrosis score. In particular, a series of LDL-PCOOH were more reflective of the severity of fibrosis score.
CONCLUSIONS: LDL-PC and LDL-PCOOH species were strongly correlated with the fibrosis score in NASH, which suggests that abnormal LDL is involved in the development of liver fibrosis.
METHODS: The serum samples from patients with NASH (female, n = 9) and SS (female, n = 4; male, n = 2) were used for isolation of LDL. Total lipids were extracted from isolated LDL, and the species of PC and PC-OOH were measured using liquid chromatography-mass spectrometry/mass spectrometry.
RESULTS: The sum of LDL-PC and the sum of LDL-PCOOH were significantly higher in NASH than in SS. Several LDL-PC (PC 32:0, 32:1, 32:2, 34:3, 36:2, sum of PC with saturated fatty acyl chains and sum of LDL-PC with polyunsaturated fatty acyl chains) and several LDL-PCOOH (34:2, 36:2, 36:3 and total) were increased significantly with increasing fibrosis score. In particular, a series of LDL-PCOOH were more reflective of the severity of fibrosis score.
CONCLUSIONS: LDL-PC and LDL-PCOOH species were strongly correlated with the fibrosis score in NASH, which suggests that abnormal LDL is involved in the development of liver fibrosis.
摘要：
背景：根据肝活检的组织学发现，非酒精性脂肪性肝病分为单纯性脂肪变性（SS）和非酒精性脂肪性肝炎（NASH）。磷脂酰胆碱(PC),磷脂的主要成分在血清脂蛋白，容易被氧化成磷脂酰胆碱过氧化氢(PC-OOH)。尽管NASH患者的低密度脂蛋白（LDL）中的脂质成分可能异常，目前还不清楚。这里,为了更好地了解NASH和SS的LDL中的脂质特征，我们比较了LDL颗粒中PC和PC-OOH种类的组成(LDL-PC，LDL-PCOOH）来自这些患者，然后阐明这些脂质与NASH严重程度之间的关联.
方法：NASH患者的血清样本（女性，n=9）和SS（女性，n=4；男性，n=2)用于分离LDL。从分离的LDL中提取总脂质，使用液相色谱-质谱/质谱法测量PC和PC-OOH的种类。
结果：NASH中LDL-PC和LDL-PCOOH的总和明显高于SS。几个LDL-PC（PC32：0，32：1，32：2，34：3，36：2，具有饱和脂肪酰基链的PC总和和具有多不饱和脂肪酰基链的LDL-PC总和）和几个LDL-PCOOH（34：2，36：2，36：3和总计）随着纤维化评分的增加而显着增加。特别是,一系列LDL-PCOOH更能反映纤维化评分的严重程度.
结论：LDL-PC和LDL-PCOOH物种与NASH的纤维化评分密切相关，这表明异常LDL参与肝纤维化的发展。
方法：NASH患者的血清样本（女性，n=9）和SS（女性，n=4；男性，n=2)用于分离LDL。从分离的LDL中提取总脂质，使用液相色谱-质谱/质谱法测量PC和PC-OOH的种类。
结果：NASH中LDL-PC和LDL-PCOOH的总和明显高于SS。几个LDL-PC（PC32：0，32：1，32：2，34：3，36：2，具有饱和脂肪酰基链的PC总和和具有多不饱和脂肪酰基链的LDL-PC总和）和几个LDL-PCOOH（34：2，36：2，36：3和总计）随着纤维化评分的增加而显着增加。特别是,一系列LDL-PCOOH更能反映纤维化评分的严重程度.
结论：LDL-PC和LDL-PCOOH物种与NASH的纤维化评分密切相关，这表明异常LDL参与肝纤维化的发展。
