Abstract：
BACKGROUND: Targeting gut dysbiosis to treat chronic diseases or to alleviate the symptoms is a new direction for medical adjuvant therapies. Recently, postbiotics have received considerable attention as they are non-viable probiotic preparations that confer various health benefits to the host without the safety problems associated with using live microbial cells.
OBJECTIVE: The aim of the study is to obtain selenium (Se) and zinc (Zn) enriched Saccharomyces boulardii postbiotic biomass and to analyze its modulation effect because these minerals play an important role in reducing gut dysbiosis linked to cardiovascular (CV) diseases.
METHODS: The effect of the S. boulardii and Se/Zn enriched yeast postbiotics on CV microbial fingerprint was studied in vitro using the gastrointestinal system (GIS 1) and analyzed by microbiological, chemical, and qPCR methods.
RESULTS: There was a 2.2 log CFU/mL increase in the total bacterial load after SeZn postbiotic treatment and in the qPCR counts of Firmicutes phyla for both treatments. Beneficial taxa, Bifidobacterium spp. and Lactobacillus spp., as well as Bacteroides spp. were up to 1.5 log higher after mineral- enriched postbiotic application, while the acetic acid level increased.
CONCLUSIONS: These preliminary studies highlight the therapeutic potential of using Se/Zn enriched yeast postbiotics as adjuvants for clinical treatments of CV diseases.
OBJECTIVE: The aim of the study is to obtain selenium (Se) and zinc (Zn) enriched Saccharomyces boulardii postbiotic biomass and to analyze its modulation effect because these minerals play an important role in reducing gut dysbiosis linked to cardiovascular (CV) diseases.
METHODS: The effect of the S. boulardii and Se/Zn enriched yeast postbiotics on CV microbial fingerprint was studied in vitro using the gastrointestinal system (GIS 1) and analyzed by microbiological, chemical, and qPCR methods.
RESULTS: There was a 2.2 log CFU/mL increase in the total bacterial load after SeZn postbiotic treatment and in the qPCR counts of Firmicutes phyla for both treatments. Beneficial taxa, Bifidobacterium spp. and Lactobacillus spp., as well as Bacteroides spp. were up to 1.5 log higher after mineral- enriched postbiotic application, while the acetic acid level increased.
CONCLUSIONS: These preliminary studies highlight the therapeutic potential of using Se/Zn enriched yeast postbiotics as adjuvants for clinical treatments of CV diseases.
摘要：
背景：靶向肠道菌群失调治疗慢性疾病或缓解症状是医学辅助治疗的新方向。最近,postbiotics已受到相当多的关注,因为它们是无活性的益生菌制剂,可为宿主提供各种健康益处,而没有与使用活微生物细胞相关的安全问题.
目的：本研究的目的是获得富含硒（Se）和锌（Zn）的布拉氏酵母菌后生物生物量，并分析其调节作用，因为这些矿物质在减少与心血管（CV）疾病相关的肠道生态失调中起着重要作用。
方法：使用胃肠道系统（GIS1）在体外研究了布拉氏酵母菌和富硒/锌酵母后生物对CV微生物指纹的影响，并通过微生物进行了分析，化学,和qPCR方法。
结果：SeZn后生物处理后的总细菌负荷和两种处理的Firmicutesphyla的qPCR计数均增加了2.2logCFU/mL。有益分类单元，双歧杆菌属。和乳杆菌属。,以及拟杆菌。在富含矿物质的后生物应用后，最高可达1.5log，而乙酸水平上升。
结论：这些初步研究强调了使用富硒/锌酵母益生菌作为临床治疗CV疾病的佐剂的治疗潜力。
目的：本研究的目的是获得富含硒（Se）和锌（Zn）的布拉氏酵母菌后生物生物量，并分析其调节作用，因为这些矿物质在减少与心血管（CV）疾病相关的肠道生态失调中起着重要作用。
方法：使用胃肠道系统（GIS1）在体外研究了布拉氏酵母菌和富硒/锌酵母后生物对CV微生物指纹的影响，并通过微生物进行了分析，化学,和qPCR方法。
结果：SeZn后生物处理后的总细菌负荷和两种处理的Firmicutesphyla的qPCR计数均增加了2.2logCFU/mL。有益分类单元，双歧杆菌属。和乳杆菌属。,以及拟杆菌。在富含矿物质的后生物应用后，最高可达1.5log，而乙酸水平上升。
结论：这些初步研究强调了使用富硒/锌酵母益生菌作为临床治疗CV疾病的佐剂的治疗潜力。
