Abstract：
Sequence-independent detection of low concentrations of nucleic acids is important for applications in forensics and diagnostics. An emission-based probe for detecting and quantifying DNA and RNA utilizing a water-soluble dicationic tetraphenylethene (TPE) derivativewas developed. The recognition is based on the electrostatic and other non-covalent interactions between the phosphate backbone of nucleic acids and the cationic probe, which cause the restriction of rotation of the aryl units of the probe, ensuing in the enhancement of the fluorescence signal. The binding was validated by different spectroscopic techniques and also by electrophoretic mobility shift assay. The probable mode of binding with the nucleic acids was studied by blind-docking studies that correlated well with the experimental results.
摘要：
低浓度核酸的序列非依赖性检测对于法医学和诊断中的应用是重要的。开发了一种基于发射的探针，用于利用水溶性双阳离子四苯基乙烯（TPE）衍生物检测和定量DNA和RNA。识别是基于核酸的磷酸骨架与阳离子探针之间的静电和其他非共价相互作用。这导致探针的芳基单元的旋转受到限制，随后荧光信号的增强。通过不同的光谱技术以及电泳迁移率变化测定来验证结合。通过与实验结果密切相关的盲对接研究研究了与核酸结合的可能模式。
