PDF(Pubmed)
Abstract：
UNASSIGNED: Antibodies against the SARS-CoV-2 spike protein are a critical immune determinant for protection against the virus. While virus neutralization is a key function of spike-specific antibodies, antibodies also mediate Fc-dependent activities that can play a role in protection or pathogenesis.
UNASSIGNED: This study characterized serum antibody responses elicited after two doses of heterologous adenovirus-vectored (Ad26/ Ad5) vaccines.
UNASSIGNED: Vaccine-induced antibody binding titers and Fc-mediated functions decreased over six months, while neutralization titers remained stable. Comparison of antibody isotypes elicited after Ad26/Ad5 vs. LNP-mRNA vaccination and after infection showed that anti-spike IgG1 were dominant and produced to high levels in all groups. The Ad26/Ad5 vaccines also induced IgG4 but not IgG2 and IgG3, whereas the LNP-mRNA vaccines elicited a full Ig spectrum (IgM, IgG1-4, IgA1-2). Convalescent COVID-19 patients had mainly IgM and IgA1 alongside IgG1. Despite these differences, the neutralization potencies against early variants were similar. However, both vaccine groups had antibodies with greater Fc potencies of binding complement and Fcg receptors than the COVID-19 group. The Ad26/Ad5 group also displayed a greater potency of RBD-specific antibody-mediated cellular phagocytosis.
UNASSIGNED: Antibodies with distinctive quality were induced by different vaccines and infection. The data imply the utility of different vaccine platforms to elicit antibody responses with fine-tuned Fc activities.
UNASSIGNED: This study characterized serum antibody responses elicited after two doses of heterologous adenovirus-vectored (Ad26/ Ad5) vaccines.
UNASSIGNED: Vaccine-induced antibody binding titers and Fc-mediated functions decreased over six months, while neutralization titers remained stable. Comparison of antibody isotypes elicited after Ad26/Ad5 vs. LNP-mRNA vaccination and after infection showed that anti-spike IgG1 were dominant and produced to high levels in all groups. The Ad26/Ad5 vaccines also induced IgG4 but not IgG2 and IgG3, whereas the LNP-mRNA vaccines elicited a full Ig spectrum (IgM, IgG1-4, IgA1-2). Convalescent COVID-19 patients had mainly IgM and IgA1 alongside IgG1. Despite these differences, the neutralization potencies against early variants were similar. However, both vaccine groups had antibodies with greater Fc potencies of binding complement and Fcg receptors than the COVID-19 group. The Ad26/Ad5 group also displayed a greater potency of RBD-specific antibody-mediated cellular phagocytosis.
UNASSIGNED: Antibodies with distinctive quality were induced by different vaccines and infection. The data imply the utility of different vaccine platforms to elicit antibody responses with fine-tuned Fc activities.
摘要：
抗SARS-CoV-2刺突蛋白的抗体是针对该病毒的保护的关键免疫决定簇。虽然病毒中和是刺突特异性抗体的关键功能，抗体还介导Fc依赖性活性，这些活性可以在保护或发病机理中发挥作用。
本研究表征了在两剂异源腺病毒载体(Ad26/Ad5)疫苗后引发的血清抗体应答。
■疫苗诱导的抗体结合滴度和Fc介导的功能在六个月内下降，而中和滴度保持稳定。Ad26/Ad5与Ad5后抗体同种型的比较LNP-mRNA疫苗接种和感染后显示，抗尖峰IgG1在所有组中占主导地位并产生高水平。Ad26/Ad5疫苗也诱导IgG4，但不诱导IgG2和IgG3，而LNP-mRNA疫苗引起完整的Ig谱（IgM，IgG1-4，IgA1-2）。恢复期COVID-19患者主要有IgM和IgA1以及IgG1。尽管存在这些差异,对早期变异的中和效力相似.然而,与COVID-19组相比,两个疫苗组的抗体具有更高的补体和Fcg受体结合Fc效力.Ad26/Ad5组还表现出更强的RBD特异性抗体介导的细胞吞噬作用。
■通过不同的疫苗和感染诱导具有独特质量的抗体。数据暗示了不同疫苗平台在具有微调的Fc活性的情况下引发抗体应答的效用。
本研究表征了在两剂异源腺病毒载体(Ad26/Ad5)疫苗后引发的血清抗体应答。
■疫苗诱导的抗体结合滴度和Fc介导的功能在六个月内下降，而中和滴度保持稳定。Ad26/Ad5与Ad5后抗体同种型的比较LNP-mRNA疫苗接种和感染后显示，抗尖峰IgG1在所有组中占主导地位并产生高水平。Ad26/Ad5疫苗也诱导IgG4，但不诱导IgG2和IgG3，而LNP-mRNA疫苗引起完整的Ig谱（IgM，IgG1-4，IgA1-2）。恢复期COVID-19患者主要有IgM和IgA1以及IgG1。尽管存在这些差异,对早期变异的中和效力相似.然而,与COVID-19组相比,两个疫苗组的抗体具有更高的补体和Fcg受体结合Fc效力.Ad26/Ad5组还表现出更强的RBD特异性抗体介导的细胞吞噬作用。
■通过不同的疫苗和感染诱导具有独特质量的抗体。数据暗示了不同疫苗平台在具有微调的Fc活性的情况下引发抗体应答的效用。
