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Abstract：
UNASSIGNED: The present study aimed to investigate whether the presence of mitoses in hyperchromatic crowded groups (HCGs) in cervical cytological specimens can serve as cytological criteria for high-grade squamous intra-epithelial lesions (HSILs).
UNASSIGNED: Various parameters were examined, including the frequency of mitotic figures per high power field (HPF) in Pap, hematoxylin eosin (HE) samples, and PHH3 immunocytochemical (ICC) and immunohistochemical (IHC) analyses.
UNASSIGNED: In the Pap and PHH3-ICC samples, the number of mitotic figures observed in HCGs was significantly higher in HSIL (P < 0.001) compared to other groups. Furthermore, the frequency of observing two or more mitoses was significantly higher in HSIL (Pap: P = 0.002, PHH3-ICC: P < 0.001) than in low-grade squamous intra-epithelial lesions (LSILs). Moreover, a comparison between Pap samples and PHH3-ICC showed that the frequency of two or more mitoses was significantly higher in the PHH3-ICC analysis of HSIL (P = 0.042). Regarding HE and PHH3-IHC samples, counting the number of mitoses in the lower and middle/upper layers of the squamous epithelial layer revealed that HSIL had a significantly higher value (HE: P = 0.0089, PHH3-IHC: P = 0.0002) than LSIL in the middle/upper layers.
UNASSIGNED: Hence, the presence of two or more mitotic figures in HCGs per HPF in cervical cytology indicates a suspicion of HSIL. The detection of mitoses in PHH3-ICC samples is more sensitive and easier to observe than in Pap samples, making it a valuable mitotic marker.
UNASSIGNED: Various parameters were examined, including the frequency of mitotic figures per high power field (HPF) in Pap, hematoxylin eosin (HE) samples, and PHH3 immunocytochemical (ICC) and immunohistochemical (IHC) analyses.
UNASSIGNED: In the Pap and PHH3-ICC samples, the number of mitotic figures observed in HCGs was significantly higher in HSIL (P < 0.001) compared to other groups. Furthermore, the frequency of observing two or more mitoses was significantly higher in HSIL (Pap: P = 0.002, PHH3-ICC: P < 0.001) than in low-grade squamous intra-epithelial lesions (LSILs). Moreover, a comparison between Pap samples and PHH3-ICC showed that the frequency of two or more mitoses was significantly higher in the PHH3-ICC analysis of HSIL (P = 0.042). Regarding HE and PHH3-IHC samples, counting the number of mitoses in the lower and middle/upper layers of the squamous epithelial layer revealed that HSIL had a significantly higher value (HE: P = 0.0089, PHH3-IHC: P = 0.0002) than LSIL in the middle/upper layers.
UNASSIGNED: Hence, the presence of two or more mitotic figures in HCGs per HPF in cervical cytology indicates a suspicion of HSIL. The detection of mitoses in PHH3-ICC samples is more sensitive and easier to observe than in Pap samples, making it a valuable mitotic marker.
摘要：
■本研究旨在调查宫颈细胞学标本中超染色拥挤组（HCG）有丝分裂的存在是否可以作为高度鳞状上皮内病变（HSIL）的细胞学标准。
■检查了各种参数，包括Pap中每个高功率场（HPF）的有丝分裂图的频率，苏木精伊红（HE）样品，和PHH3免疫细胞化学（ICC）和免疫组织化学（IHC）分析。
■在Pap和PHH3-ICC样本中，与其他组相比，HSIL中HCG中观察到的有丝分裂数量显着增加（P<0.001）。此外，在HSIL(Pap:P=0.002,PHH3-ICC:P<0.001)中观察到两个或两个以上有丝分裂的频率显著高于低度鳞状上皮内病变(LSILs).此外，Pap样本和PHH3-ICC之间的比较显示,在HSIL的PHH3-ICC分析中,两种或两种以上有丝分裂的频率显著较高(P=0.042).关于HE和PHH3-IHC样本，计算鳞状上皮层下层和中/上层的有丝分裂数量，发现HSIL的值（HE：P=0.0089，PHH3-IHC：P=0.0002）明显高于中/上层的LSIL。
■因此，在宫颈细胞学检查中,每个HPF的HCG中存在两个或更多有丝分裂图,表明怀疑HSIL.PHH3-ICC样品中有丝分裂的检测比Pap样品更灵敏，更容易观察，使其成为有价值的有丝分裂标记。
■检查了各种参数，包括Pap中每个高功率场（HPF）的有丝分裂图的频率，苏木精伊红（HE）样品，和PHH3免疫细胞化学（ICC）和免疫组织化学（IHC）分析。
■在Pap和PHH3-ICC样本中，与其他组相比，HSIL中HCG中观察到的有丝分裂数量显着增加（P<0.001）。此外，在HSIL(Pap:P=0.002,PHH3-ICC:P<0.001)中观察到两个或两个以上有丝分裂的频率显著高于低度鳞状上皮内病变(LSILs).此外，Pap样本和PHH3-ICC之间的比较显示,在HSIL的PHH3-ICC分析中,两种或两种以上有丝分裂的频率显著较高(P=0.042).关于HE和PHH3-IHC样本，计算鳞状上皮层下层和中/上层的有丝分裂数量，发现HSIL的值（HE：P=0.0089，PHH3-IHC：P=0.0002）明显高于中/上层的LSIL。
■因此，在宫颈细胞学检查中,每个HPF的HCG中存在两个或更多有丝分裂图,表明怀疑HSIL.PHH3-ICC样品中有丝分裂的检测比Pap样品更灵敏，更容易观察，使其成为有价值的有丝分裂标记。
