PDF(Pubmed)
Abstract：
UNASSIGNED: It\'s crucial to identify an easily detectable biomarker that is specific to radiation injury in order to effectively classify injured individuals in the early stage in large-scale nuclear accidents.
UNASSIGNED: C57BL/6J mice were subjected to whole-body and partial-body γ irradiation, as well as whole-body X-ray irradiation to explore the response of serum sSelectin-L to radiation injury. Then, it was compared with its response to lipopolysaccharide-induced acute infection and doxorubicin-induced DNA damage to study the specificity of sSelectin-L response to radiation. Furthermore, it was further evaluated in serum samples from nasopharyngeal carcinoma patients before and after radiotherapy. Simulated rescue experiments using Amifostine or bone marrow transplantation were conducted in mice with acute radiation syndrome to determine the potential for establishing sSelectin-L as a prognostic marker. The levels of sSelectin-L were dynamically measured using the ELISA method.
UNASSIGNED: Selectin-L is mainly expressed in hematopoietic tissues and lymphatic tissues. Mouse sSelectin-L showed a dose-dependent decrease from 1 day after irradiation and exhibited a positive correlation with lymphocyte counts. Furthermore, the level of sSelectin-L reflected the degree of radiation injury in partial-body irradiation mice and in nasopharyngeal carcinoma patients. sSelectin-L was closely related to the total dose of γ or X ray. There was no significant change in the sSelectin-L levels in mice intraperitoneal injected with lipopolysaccharide or doxorubicin. The sSelectin-L was decreased slower and recovered faster than lymphocyte count in acute radiation syndrome mice treated with Amifostine or bone marrow transplantation.
UNASSIGNED: Our study shows that sSelectin-L has the potential to be an early biomarker to classify injured individuals after radiation accidents, and to be a prognostic indicator of successful rescue of radiation victims.
UNASSIGNED: C57BL/6J mice were subjected to whole-body and partial-body γ irradiation, as well as whole-body X-ray irradiation to explore the response of serum sSelectin-L to radiation injury. Then, it was compared with its response to lipopolysaccharide-induced acute infection and doxorubicin-induced DNA damage to study the specificity of sSelectin-L response to radiation. Furthermore, it was further evaluated in serum samples from nasopharyngeal carcinoma patients before and after radiotherapy. Simulated rescue experiments using Amifostine or bone marrow transplantation were conducted in mice with acute radiation syndrome to determine the potential for establishing sSelectin-L as a prognostic marker. The levels of sSelectin-L were dynamically measured using the ELISA method.
UNASSIGNED: Selectin-L is mainly expressed in hematopoietic tissues and lymphatic tissues. Mouse sSelectin-L showed a dose-dependent decrease from 1 day after irradiation and exhibited a positive correlation with lymphocyte counts. Furthermore, the level of sSelectin-L reflected the degree of radiation injury in partial-body irradiation mice and in nasopharyngeal carcinoma patients. sSelectin-L was closely related to the total dose of γ or X ray. There was no significant change in the sSelectin-L levels in mice intraperitoneal injected with lipopolysaccharide or doxorubicin. The sSelectin-L was decreased slower and recovered faster than lymphocyte count in acute radiation syndrome mice treated with Amifostine or bone marrow transplantation.
UNASSIGNED: Our study shows that sSelectin-L has the potential to be an early biomarker to classify injured individuals after radiation accidents, and to be a prognostic indicator of successful rescue of radiation victims.
摘要：
■为了在大规模核事故的早期阶段对受伤的个体进行有效分类，确定一种易于检测的特定于辐射损伤的生物标志物至关重要。
■C57BL/6J小鼠接受全身和局部γ射线照射，以及全身X射线照射，以探讨血清sSelectin-L对辐射损伤的反应。然后，将其与对脂多糖诱导的急性感染和阿霉素诱导的DNA损伤的反应进行比较，以研究sSelectin-L对辐射的反应的特异性。此外，在鼻咽癌患者放疗前后的血清样本中进一步评估.在患有急性放射综合征的小鼠中进行了使用氨磷汀或骨髓移植的模拟抢救实验，以确定建立sSelectin-L作为预后标志物的潜力。使用ELISA方法动态测量sSelectin-L的水平。
■选择素-L主要在造血组织和淋巴组织中表达。小鼠sSelectin-L从照射后1天开始显示出剂量依赖性降低，并与淋巴细胞计数呈正相关。此外，sSelectin-L的水平反映了部分身体照射小鼠和鼻咽癌患者的放射损伤程度。sSelectin-L与γ或X射线的总剂量密切相关。腹膜内注射脂多糖或阿霉素的小鼠sSelectin-L水平没有显着变化。在用氨磷汀或骨髓移植治疗的急性放射综合征小鼠中，sSelectin-L比淋巴细胞计数降低更慢，恢复更快。
■我们的研究表明，sSelectin-L有可能成为对辐射事故后受伤个体进行分类的早期生物标志物，并成为成功抢救辐射受害者的预后指标。
■C57BL/6J小鼠接受全身和局部γ射线照射，以及全身X射线照射，以探讨血清sSelectin-L对辐射损伤的反应。然后，将其与对脂多糖诱导的急性感染和阿霉素诱导的DNA损伤的反应进行比较，以研究sSelectin-L对辐射的反应的特异性。此外，在鼻咽癌患者放疗前后的血清样本中进一步评估.在患有急性放射综合征的小鼠中进行了使用氨磷汀或骨髓移植的模拟抢救实验，以确定建立sSelectin-L作为预后标志物的潜力。使用ELISA方法动态测量sSelectin-L的水平。
■选择素-L主要在造血组织和淋巴组织中表达。小鼠sSelectin-L从照射后1天开始显示出剂量依赖性降低，并与淋巴细胞计数呈正相关。此外，sSelectin-L的水平反映了部分身体照射小鼠和鼻咽癌患者的放射损伤程度。sSelectin-L与γ或X射线的总剂量密切相关。腹膜内注射脂多糖或阿霉素的小鼠sSelectin-L水平没有显着变化。在用氨磷汀或骨髓移植治疗的急性放射综合征小鼠中，sSelectin-L比淋巴细胞计数降低更慢，恢复更快。
■我们的研究表明，sSelectin-L有可能成为对辐射事故后受伤个体进行分类的早期生物标志物，并成为成功抢救辐射受害者的预后指标。
