Abstract：
Due to its non-invasive nature and easy accessibility, urine serves as a convenient bi-ological fluid for research purposes. Furthermore, urine samples are uncomplicated to preserve and relatively inexpensive. MicroRNA (miRNAs), small molecules that regulate gene expression post-transcriptionally, play vital roles in numerous cellular processes, including apoptosis, cell differentiation, development, and proliferation. Their dysregulated expression in urine has been proposed as a potential bi-omarker for various human diseases, including bladder cancer. To draw reliable conclusions about the roles of urinary miRNAs in human diseases, it is essential to have dependable and reproducible methods for miRNA extraction and profiling. In this review, we address the technical challenges associated with studying urinary miRNAs and provide an update on the current technologies used for urinary miRNA isolation, quality control assessment, and miRNA profiling, highlighting both their advantages and limitations.
摘要：
由于其非侵入性和易于访问的性质，尿液作为研究目的的方便的生物流体。此外，尿液样本保存起来并不复杂，而且相对便宜。微小RNA(miRNA),转录后调节基因表达的小分子，在许多细胞过程中发挥重要作用，包括细胞凋亡,细胞分化，发展,和扩散。它们在尿液中的失调表达已被认为是各种人类疾病的潜在标志，包括膀胱癌.为了得出关于尿液miRNAs在人类疾病中的作用的可靠结论，有可靠和可重复的miRNA提取和谱分析方法是至关重要的.在这次审查中,我们解决了与研究尿miRNA相关的技术挑战，并提供了用于尿miRNA分离的当前技术的更新。质量控制评估，和miRNA分析，强调它们的优点和局限性。
