Abstract：
Mycotoxins and pesticides frequently coexist in agricultural commodities on a global scale. The potential transgenerational consequences induced by these substances pose a significant threat to human health. However, there is a lack of data concerning the effects of co-contamination by these chemicals in the F1 generation following parental exposure. This investigation delved into the mixture effects of T-2 toxin (T-2) and epoxiconazole (EPO) on the offspring of zebrafish (Danio rerio). The findings revealed that exposure across generations to a combination of T-2 and EPO resulted in toxicity in the larvae of the F1 generation. This was demonstrated by a significant increase in the levels or activities of malondialdehyde (MDA), thyroxine (T4), Caspase3, and cas9, along with a decrease in the levels of cyp19a, ERα, and ERβ. These outcomes suggested that cross-generational exposure to T-2 and EPO in D. rerio disrupted oxidative balance, induced cell apoptosis, and affected the endocrine system. Moreover, these effects were magnified when the F1 generation was continuously exposed to these compounds. Notably, these adverse effects could persist in subsequent generations without additional exposure. This study underscored the potential dangers associated with the simultaneous presence of T-2 and EPO on the development of fish offspring and the resulting environmental hazards to aquatic ecosystems. These findings emphasized the significant health risks posed by cross-generational exposure and highlighted the need for additional legislative measures to address these concerns.
摘要：
霉菌毒素和农药经常在全球范围内共存于农产品中。这些物质引起的潜在跨代后果对人类健康构成重大威胁。然而,缺乏有关父母暴露后F1代中这些化学物质共同污染影响的数据。这项研究探讨了T-2毒素（T-2）和环氧康唑（EPO）对斑马鱼（Daniorerio）后代的混合作用。研究结果表明，跨代暴露于T-2和EPO的组合会导致F1代幼虫的毒性。丙二醛（MDA）的水平或活性显着增加，甲状腺素(T4),Caspase3和cas9，以及cyp19a水平的下降，ERα,和ERβ。这些结果表明，跨世代接触T-2和EPO在D.rerio破坏了氧化平衡，诱导细胞凋亡，影响内分泌系统.此外，当F1代连续暴露于这些化合物时，这些影响被放大。值得注意的是,这些不良反应可能会持续到后代,而无需额外暴露.这项研究强调了T-2和EPO同时存在对鱼类后代发育的潜在危险以及由此对水生生态系统的环境危害。这些调查结果强调了跨代接触带来的重大健康风险，并强调需要采取额外的立法措施来解决这些问题。
