Abstract：
Cordiera myrciifolia is an abundant species in Northeast Brazil that presents metabolites of biological/therapeutic interest. From this perspective, the present study aimed to investigate the chemical constituents and evaluate the in vitro antimicrobial activity of hexane (HECM) and ethanolic (EECM) extracts of C. myrciifolia leaves. The extracts were analyzed by chromatographic techniques (GC and UPLC) coupled with mass spectrometry. The antimicrobial activity of the extracts and the extracts combined with conventional drugs was evaluated by microdilution. The in vitro effect of the treatments on Candida\'s morphological transition was verified through cultivation in humid chambers. In HECM, 11 constituents including fatty acids, and triterpenes, including phytosterols, alkanes, tocols, and primary alcohols were identified. Triterpenes represented >40% of the identified constituents, with Lupeol being the most representative. In EECM, 13 constituents were identified, of which eight belonged to the class of flavonoids. High antibacterial activity of HECM was detected against Escherichia coli and Staphylococcus aureus, with Minimum Inhibitory Concentrations of 8 and 16 μg/mL, respectively. The combined activity was more effective when combined with Norfloxacin and Imipenem. In anti-Candida activity, the IC50 of the extracts ranged from 36.6 to 129.1 μg/mL. There was potentiating effect when associated with Fluconazole. Both extracts inhibited the filamentous growth of C. tropicalis at a concentration of 512 μg/mL. C. myrciifolia extracts prove to be candidates for the development of new therapeutic formulations to treat bacterial and fungal infections.
摘要：
桃金娘是巴西东北部的丰富物种，具有生物学/治疗意义的代谢产物。从这个角度来看,本研究旨在研究桃金娘叶正己烷（HECM）和乙醇（EECM）提取物的化学成分并评估其体外抗菌活性。通过与质谱联用的色谱技术(GC和UPLC)分析提取物。通过微量稀释评估提取物和与常规药物组合的提取物的抗微生物活性。通过在潮湿的室内培养，验证了处理对念珠菌形态转变的体外影响。在HECM中，11种成分，包括脂肪酸，和三萜,包括植物甾醇,烷烃,,并鉴定了伯醇。三萜代表>40%的确定的成分，Lupeol是最具代表性的。在EECM中，确定了13种成分，其中八种属于类黄酮。HECM对大肠杆菌和金黄色葡萄球菌具有较高的抗菌活性,最低抑制浓度为8和16μg/mL，分别。与诺氟沙星和亚胺培南联合使用时，联合活性更有效。在抗念珠菌活性方面,提取物的IC50范围为36.6至129.1μg/mL。当与氟康唑结合时，有增强作用。两种提取物在512μg/mL的浓度下都抑制热带假丝酵母的丝状生长。金花芽孢杆菌提取物被证明是用于开发新的治疗性制剂以治疗细菌和真菌感染的候选物。
