Abstract：
Organophosphorus flame retardants, such as triphenyl phosphate (TPhP), exist ubiquitously in various environments owing to their widespread usage. Potential toxic effects of residual flame retardants on cultured non-fish species are not concerned commonly. TPhP-induced physiological and biochemical effects in an aquatic turtle were evaluated here by systematically investigating the changes in growth and locomotor performance, hepatic antioxidant ability and metabolite, and intestinal microbiota composition of turtle hatchlings after exposure to different TPhP concentrations. Reduced locomotor ability and antioxidant activity were only observed in the highest concentration group. Several metabolic perturbations that involved in amino acid, energy and nucleotide metabolism, in exposed turtles were revealed by metabolite profiles. No significant among-group difference in intestinal bacterial diversity was observed, but the composition was changed markedly in exposed turtles. Increased relative abundances of some bacterial genera (e.g., Staphylococcus, Vogesella and Lawsonella) probably indicated adverse outcomes of TPhP exposure. Despite having only limited impacts of exposure at environmentally relevant levels, our results revealed potential ecotoxicological risks of residual TPhP for aquatic turtles considering TPhP-induced metabolic perturbations and intestinal bacterial changes.
摘要：
有机磷阻燃剂，如磷酸三苯酯（TPhP），由于它们的广泛使用，在各种环境中普遍存在。残留阻燃剂对养殖非鱼类物种的潜在毒性作用并不常见。通过系统地研究生长和运动性能的变化，评估了TPhP在水生乌龟中诱导的生理和生化作用，肝脏抗氧化能力和代谢产物，暴露于不同TPhP浓度后，乌龟幼体的肠道菌群组成。仅在最高浓度组中观察到运动能力和抗氧化活性降低。涉及氨基酸的几种代谢扰动，能量和核苷酸代谢，在暴露的海龟中，代谢物谱显示。肠道细菌多样性没有观察到显著的组间差异，但是暴露的海龟的成分发生了明显的变化。某些细菌属的相对丰度增加(例如，葡萄球菌,Vogesella和Lawsonella）可能表明TPhP暴露的不良后果。尽管暴露在环境相关水平上的影响有限，我们的结果揭示了考虑到TPhP诱导的代谢扰动和肠道细菌变化,水龟残留TPhP的潜在生态毒理学风险.
