Abstract：
As an emerging nanoprobe, surface enhanced Raman scattering (SERS) tags hold significant promise in sensing and bioimaging applications due to their attractive merits of anti-photobleaching ability, high sensitivity and specificity, multiplex, and low background capabilities. Recently, several reviews have proposed the application of SERS tags in different fields, however, the specific sensing strategies of SERS tags-based sensors for cellular molecules have not yet been systematically summarized. To provide beneficial and comprehensive insights into the advanced SERS tags technique at the cellular level, this review systematically elaborated on the latest advances in SERS tags-based sensors for cellular molecules detection and imaging. The general SERS tags-based sensing strategies for biomolecules and ions were first introduced according to molecular classes. Then, aiming at such molecules located in the extracellular, cellular membrane and intracellular regions, the tailored strategies by designing and manipulating SERS tags were summarized and explored through several key examples. Finally, the challenges and perspectives of developing high performance of advanced SERS tags were briefly discussed to provide effective guidance for further development and extended applications.
摘要：
作为一种新兴的纳米探针，表面增强拉曼散射（SERS）标签在传感和生物成像应用中具有重要的前景，因为它们具有抗光漂白能力的诱人优点，高灵敏度和特异性，多路复用，背景能力低。最近,一些综述提出了SERS标签在不同领域的应用，然而,基于SERS标签的细胞分子传感器的具体传感策略尚未得到系统总结.为了在细胞水平上对先进的SERS标签技术提供有益和全面的见解，本文系统阐述了基于SERS标签的细胞分子检测和成像传感器的最新进展。首先根据分子类别介绍了用于生物分子和离子的基于一般SERS标签的传感策略。然后，针对这些位于细胞外的分子，细胞膜和细胞内区域，通过几个关键示例，总结和探索了通过设计和操纵SERS标签的定制策略。最后,简要讨论了开发高性能高级SERS标签的挑战和前景，为进一步开发和扩展应用提供有效指导。
