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Abstract：
Conventional views associate microbial biofilm with demineralization in root caries (RC) onset, while research on their collagenases role in the breakdown of collagen matrix has been sporadically developed, primarily in vitro. Recent discoveries, however, reveal proteolytic bacteria enrichment, specially Porphyromonas and other periodontitis-associated bacteria in subgingivally extended lesions, suggesting a potential role in RC by the catabolism of dentin organic matrix. Moreover, genes encoding proteases and bacterial collagenases, including the U32 family collagenases, were found to be overexpressed in both coronal and root dentinal caries. Despite these advancements, to prove microbial collagenolytic proteases\' definitive role in RC remains a significant challenge. A more thorough investigation is warranted to explore the potential of anti-collagenolytic agents in modulating biofilm metabolic processes or inhibiting/reducing the size of RC lesions. Prospective treatments targeting collagenases and promoting biomodification through collagen fibril cross-linking show promise for RC prevention and management. However, these studies are currently in the in vitro phase, necessitating additional research to translate findings into clinical applications. This is a comprehensive state-of-the-art review aimed to explore contributing factors to the formation of RC lesions, particularly focusing on collagen degradation in root tissues by microbial collagenases.
摘要：
传统观点将微生物生物膜与根龋（RC）发作中的脱矿质联系起来，虽然关于它们的胶原酶在胶原基质分解中的作用的研究已经零星地发展起来，主要是在体外。最近的发现,然而,揭示蛋白水解细菌的富集，特别是卟啉单胞菌和其他牙周炎相关细菌在牙龈下扩展病变，提示牙本质有机基质的分解代谢在RC中的潜在作用。此外，编码蛋白酶和细菌胶原酶的基因，包括U32家族胶原酶,发现在冠状和根牙本质龋齿中均过表达。尽管取得了这些进步,证明微生物胶原酶在RC中的决定性作用仍然是一个重大挑战。有必要进行更彻底的研究，以探索抗胶原分解剂在调节生物膜代谢过程或抑制/减少RC病变大小方面的潜力。针对胶原酶和通过胶原纤维交联促进生物修饰的前瞻性治疗显示出预防和管理RC的希望。然而,这些研究目前处于体外阶段，需要进行额外的研究以将发现转化为临床应用。这是一项全面的最新综述，旨在探索RC病变形成的因素，特别关注微生物胶原酶在根组织中的胶原降解。
