Abstract：
The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. In this sensing system, AFB1 and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, and were used as recognition probes to specifically capture the target. Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB1 with limits of 426.18 and 79.55 fg mL-1 for AFB1 and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
摘要：
一个简单的发展，快速,同时检测霉菌毒素的灵敏技术对保障食品和药品的安全具有重要意义。在这里，开发了一种具有高灵敏度和重现性的荧光传感器，用于同时检测黄曲霉毒素B1（AFB1）和曲霉毒素A（OTA）。在这个传感系统中，AFB1和OTA适体通过互补碱基配对原理共固定在磁珠(MBs)表面,形成Y形结构,并用作识别探针以特异性捕获目标。通过用于原子转移自由基聚合的电子转移再生的活化剂(ARGETATRP)被用作信号放大策略以提高灵敏度。在抗体末端修饰的引发剂引发ARGETATRP反应。设计了不同的荧光信号，实现了OTA和AFB1的同时检测，AFB1和OTA的限为426.18和79.55fgmL-1，分别。此外,对三种类型的样品进行了实验，两种真菌毒素的回收率在87.30%至109.50%之间，在可重复条件下，相对标准偏差范围为0.50％至4.92％。结果表明,所开发的aptasensor足以满足两种真菌毒素在食品药品安全方面的不同监管要求,显示出巨大的潜力。
