PDF(Pubmed)
Abstract：
UNASSIGNED: Lymphomas are among the most important and common malignant tumors in cats. Differentiating lymphomas from reactive lymphoid proliferations can be challenging, so additional tools such as clonality assessment by PCR are important in diagnosis finding. Several PCR assays have been developed to assess clonality in feline lymphomas. For T-cell lymphomas TRG (T-cell receptor gamma) genes are the preferred target whereas for B-cell lymphomas most primer sets target immunoglobulin heavy chain (IGH) genes. Here we compare commonly used diagnostic primer sets for the assessment of clonality in feline lymphomas under controlled conditions (i.e., identical sample set, PCR setup, amplicon detection system).
UNASSIGNED: Formalin-fixed and paraffin-embedded samples from 31 feline T-cell lymphomas, 29 B-cell lymphomas, and 11 non-neoplastic controls were analyzed by PCR combined with capillary electrophoresis.
UNASSIGNED: We show that the combination of the primer sets published by Weiss et al. and Mochizuki et al. provided the best results for T-cell clonality, i.e., correctly assigns most populations as clonal or polyclonal. For B-cell clonality, the combination of the primer sets by Mochizuki et al. and Rout et al. gave the best results when omitting the Kde gene rearrangement due to its low specificity. This study rigorously evaluated various primer sets under uniform experimental conditions to improve accuracy of lymphoma diagnostic and provides a recommendation for achieving the highest diagnostic precision in lymphoma clonality analysis.
UNASSIGNED: Formalin-fixed and paraffin-embedded samples from 31 feline T-cell lymphomas, 29 B-cell lymphomas, and 11 non-neoplastic controls were analyzed by PCR combined with capillary electrophoresis.
UNASSIGNED: We show that the combination of the primer sets published by Weiss et al. and Mochizuki et al. provided the best results for T-cell clonality, i.e., correctly assigns most populations as clonal or polyclonal. For B-cell clonality, the combination of the primer sets by Mochizuki et al. and Rout et al. gave the best results when omitting the Kde gene rearrangement due to its low specificity. This study rigorously evaluated various primer sets under uniform experimental conditions to improve accuracy of lymphoma diagnostic and provides a recommendation for achieving the highest diagnostic precision in lymphoma clonality analysis.
摘要：
■淋巴瘤是猫中最重要和最常见的恶性肿瘤之一。区分淋巴瘤和反应性淋巴增生可能是具有挑战性的，因此,通过PCR进行克隆性评估等其他工具在诊断发现中非常重要.已经开发了几种PCR测定法来评估猫淋巴瘤的克隆性。对于T细胞淋巴瘤,TRG(T细胞受体γ)基因是优选的靶,而对于B细胞淋巴瘤,大多数引物设定靶免疫球蛋白重链(IGH)基因。在这里，我们比较了在受控条件下评估猫淋巴瘤克隆性的常用诊断引物集（即，相同的样品组,PCR设置，扩增子检测系统)。
■来自31个猫科动物T细胞淋巴瘤的福尔马林固定和石蜡包埋样品，29个B细胞淋巴瘤，并通过PCR结合毛细管电泳分析了11例非肿瘤对照。
■我们表明Weiss等人发表的引物组的组合。和Mochizuki等人。为T细胞克隆性提供了最好的结果，即，正确地将大多数种群分配为克隆或多克隆。对于B细胞克隆性，Mochizuki等人的引物集的组合。和Rout等人。当省略Kde基因重排时，由于其低特异性，给出了最好的结果。这项研究在统一的实验条件下严格评估了各种引物集，以提高淋巴瘤诊断的准确性，并为在淋巴瘤克隆性分析中实现最高诊断精度提供了建议。
■来自31个猫科动物T细胞淋巴瘤的福尔马林固定和石蜡包埋样品，29个B细胞淋巴瘤，并通过PCR结合毛细管电泳分析了11例非肿瘤对照。
■我们表明Weiss等人发表的引物组的组合。和Mochizuki等人。为T细胞克隆性提供了最好的结果，即，正确地将大多数种群分配为克隆或多克隆。对于B细胞克隆性，Mochizuki等人的引物集的组合。和Rout等人。当省略Kde基因重排时，由于其低特异性，给出了最好的结果。这项研究在统一的实验条件下严格评估了各种引物集，以提高淋巴瘤诊断的准确性，并为在淋巴瘤克隆性分析中实现最高诊断精度提供了建议。
