PDF(Pubmed)
Abstract：
UNASSIGNED: Traditional microbiological detection methods used to detect pulmonary infections in people living with HIV (PLHIV) are usually time-consuming and have low sensitivity, leading to delayed treatment. We aimed to evaluate the diagnostic value of metagenomics next-generation sequencing (mNGS) for microbial diagnosis of suspected pulmonary infections in PLHIV.
UNASSIGNED: We retrospectively analyzed PLHIV who were hospitalized due to suspected pulmonary infections at the sixth people hospital of Zhengzhou from November 1, 2021 to June 30, 2022. Bronchoalveolar lavage fluid (BALF) samples of PLHIV were collected and subjected to routine microbiological examination and mNGS detection. The diagnostic performance of the two methods was compared to evaluate the diagnostic value of mNGS for unknown pathogens.
UNASSIGNED: This study included a total of 36 PLHIV with suspected pulmonary infections, of which 31 were male. The reporting period of mNGS is significantly shorter than that of CMTs. The mNGS positive rate of BALF samples in PLHIV was 83.33%, which was significantly higher than that of smear and culture (44.4%, P<0.001). In addition, 11 patients showed consistent results between the two methods. Futhermore, mNGS showed excellent performance in identifying multi-infections in PLHIV, and 27 pathogens were detected in the BALF of 30 PLHIV by mNGS, among which 15 PLHIV were found to have multiple microbial infections (at least 3 pathogens). Pneumocystis jirovecii, human herpesvirus type 5, and human herpesvirus type 4 were the most common pathogen types.
UNASSIGNED: For PLHIV with suspected pulmonary infections, mNGS is capable of rapidly and accurately identifying the pathogen causing the pulmonary infection, which contributes to implement timely and accurate anti-infective treatment.
UNASSIGNED: We retrospectively analyzed PLHIV who were hospitalized due to suspected pulmonary infections at the sixth people hospital of Zhengzhou from November 1, 2021 to June 30, 2022. Bronchoalveolar lavage fluid (BALF) samples of PLHIV were collected and subjected to routine microbiological examination and mNGS detection. The diagnostic performance of the two methods was compared to evaluate the diagnostic value of mNGS for unknown pathogens.
UNASSIGNED: This study included a total of 36 PLHIV with suspected pulmonary infections, of which 31 were male. The reporting period of mNGS is significantly shorter than that of CMTs. The mNGS positive rate of BALF samples in PLHIV was 83.33%, which was significantly higher than that of smear and culture (44.4%, P<0.001). In addition, 11 patients showed consistent results between the two methods. Futhermore, mNGS showed excellent performance in identifying multi-infections in PLHIV, and 27 pathogens were detected in the BALF of 30 PLHIV by mNGS, among which 15 PLHIV were found to have multiple microbial infections (at least 3 pathogens). Pneumocystis jirovecii, human herpesvirus type 5, and human herpesvirus type 4 were the most common pathogen types.
UNASSIGNED: For PLHIV with suspected pulmonary infections, mNGS is capable of rapidly and accurately identifying the pathogen causing the pulmonary infection, which contributes to implement timely and accurate anti-infective treatment.
摘要：
■用于检测HIV感染者（PLHIV）肺部感染的传统微生物检测方法通常耗时且灵敏度低，导致延迟治疗。我们旨在评估宏基因组学下一代测序（mNGS）对PLHIV中可疑肺部感染的微生物诊断的诊断价值。
我们回顾性分析了2021年11月1日至2022年6月30日在郑州市第六人民医院因疑似肺部感染而住院的PLHIV。收集PLHIV的支气管肺泡灌洗液（BALF）样品，并进行常规微生物学检查和mNGS检测。比较两种方法的诊断性能，以评估mNGS对未知病原体的诊断价值。
■本研究共纳入36例疑似肺部感染的PLHIV,其中31人为男性。mNGS的报告期明显短于CMT的报告期。PLHIV中BALF样本的mNGS阳性率为83.33%,显著高于涂片和培养(44.4%，P<0.001)。此外,11例患者在两种方法之间显示一致的结果。Futhermore,mNGS在识别PLHIV中的多重感染方面表现出优异的性能，通过mNGS在30例PLHIV的BALF中检测到27种病原体，其中15例PLHIV被发现有多种微生物感染（至少3种病原体）。肺孢子虫jirovecii,人疱疹病毒5型和人疱疹病毒4型是最常见的病原体类型。
■对于疑似肺部感染的PLHIV，mNGS能够快速准确地识别引起肺部感染的病原体，有助于实施及时、准确的抗感染治疗。
我们回顾性分析了2021年11月1日至2022年6月30日在郑州市第六人民医院因疑似肺部感染而住院的PLHIV。收集PLHIV的支气管肺泡灌洗液（BALF）样品，并进行常规微生物学检查和mNGS检测。比较两种方法的诊断性能，以评估mNGS对未知病原体的诊断价值。
■本研究共纳入36例疑似肺部感染的PLHIV,其中31人为男性。mNGS的报告期明显短于CMT的报告期。PLHIV中BALF样本的mNGS阳性率为83.33%,显著高于涂片和培养(44.4%，P<0.001)。此外,11例患者在两种方法之间显示一致的结果。Futhermore,mNGS在识别PLHIV中的多重感染方面表现出优异的性能，通过mNGS在30例PLHIV的BALF中检测到27种病原体，其中15例PLHIV被发现有多种微生物感染（至少3种病原体）。肺孢子虫jirovecii,人疱疹病毒5型和人疱疹病毒4型是最常见的病原体类型。
■对于疑似肺部感染的PLHIV，mNGS能够快速准确地识别引起肺部感染的病原体，有助于实施及时、准确的抗感染治疗。
