PDF(Pubmed)
Abstract：
UNASSIGNED: The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) strains has underscored the urgent need for novel therapeutic approaches. Carbon-based nanomaterials, such as graphene oxide (GO), have shown potential in anti-TB activities but suffer from significant toxicity issues.
UNASSIGNED: This study explores the anti-TB potential of differently functionalized graphene quantum dots (GQDs) - non-functionalized, L-GQDs, aminated (NH2-GQDs), and carboxylated (COOH-GQDs) - alone and in combination with standard TB drugs (isoniazid, amikacin, and linezolid). Their effects were assessed in both axenic cultures and in vitro infection models.
UNASSIGNED: GQDs alone did not demonstrate direct mycobactericidal effects nor trapping activity. However, the combination of NH2-GQDs with amikacin significantly reduced CFUs in in vitro models. NH2-GQDs and COOH-GQDs also enhanced the antimicrobial activity of amikacin in infected macrophages, although L-GQDs and COOH-GQDs alone showed no significant activity.
UNASSIGNED: The results suggest that specific types of GQDs, particularly NH2-GQDs, can enhance the efficacy of existing anti-TB drugs. These nanoparticles might serve as effective adjuvants in anti-TB therapy by boosting drug performance and reducing bacterial counts in host cells, highlighting their potential as part of advanced drug delivery systems in tuberculosis treatment. Further investigations are needed to better understand their mechanisms and optimize their use in clinical settings.
UNASSIGNED: This study explores the anti-TB potential of differently functionalized graphene quantum dots (GQDs) - non-functionalized, L-GQDs, aminated (NH2-GQDs), and carboxylated (COOH-GQDs) - alone and in combination with standard TB drugs (isoniazid, amikacin, and linezolid). Their effects were assessed in both axenic cultures and in vitro infection models.
UNASSIGNED: GQDs alone did not demonstrate direct mycobactericidal effects nor trapping activity. However, the combination of NH2-GQDs with amikacin significantly reduced CFUs in in vitro models. NH2-GQDs and COOH-GQDs also enhanced the antimicrobial activity of amikacin in infected macrophages, although L-GQDs and COOH-GQDs alone showed no significant activity.
UNASSIGNED: The results suggest that specific types of GQDs, particularly NH2-GQDs, can enhance the efficacy of existing anti-TB drugs. These nanoparticles might serve as effective adjuvants in anti-TB therapy by boosting drug performance and reducing bacterial counts in host cells, highlighting their potential as part of advanced drug delivery systems in tuberculosis treatment. Further investigations are needed to better understand their mechanisms and optimize their use in clinical settings.
摘要：
■耐药结核分枝杆菌（Mtb）菌株的出现强调了对新治疗方法的迫切需要。碳基纳米材料,如氧化石墨烯(GO)，已经显示出潜在的抗TB活性，但遭受显著的毒性问题。
■这项研究探索了不同功能化的石墨烯量子点（GQDs）的抗结核病潜力-非功能化，L-GQD,胺化（NH2-GQDs），和羧化（COOH-GQDs）-单独和与标准结核病药物联合使用（异烟肼，阿米卡星，和利奈唑胺）。在无菌培养和体外感染模型中评估了它们的作用。
■单独的GQD没有表现出直接的分枝杆菌杀菌作用或捕获活性。然而,在体外模型中，NH2-GQDs与阿米卡星的组合显着降低了CFU。NH2-GQDs和COOH-GQDs也增强了阿米卡星在感染的巨噬细胞中的抗菌活性，虽然L-GQDs和COOH-GQDs单独没有显示出显著的活性。
■结果表明，特定类型的GQDs，特别是NH2-GQDs，可以增强现有抗结核药物的疗效。这些纳米颗粒可能通过提高药物性能和减少宿主细胞中的细菌计数，作为抗结核治疗的有效佐剂。强调它们作为结核病治疗中先进药物输送系统的一部分的潜力。需要进一步的研究以更好地了解其机制并优化其在临床环境中的使用。
■这项研究探索了不同功能化的石墨烯量子点（GQDs）的抗结核病潜力-非功能化，L-GQD,胺化（NH2-GQDs），和羧化（COOH-GQDs）-单独和与标准结核病药物联合使用（异烟肼，阿米卡星，和利奈唑胺）。在无菌培养和体外感染模型中评估了它们的作用。
■单独的GQD没有表现出直接的分枝杆菌杀菌作用或捕获活性。然而,在体外模型中，NH2-GQDs与阿米卡星的组合显着降低了CFU。NH2-GQDs和COOH-GQDs也增强了阿米卡星在感染的巨噬细胞中的抗菌活性，虽然L-GQDs和COOH-GQDs单独没有显示出显著的活性。
■结果表明，特定类型的GQDs，特别是NH2-GQDs，可以增强现有抗结核药物的疗效。这些纳米颗粒可能通过提高药物性能和减少宿主细胞中的细菌计数，作为抗结核治疗的有效佐剂。强调它们作为结核病治疗中先进药物输送系统的一部分的潜力。需要进一步的研究以更好地了解其机制并优化其在临床环境中的使用。
