PDF(Pubmed)
Abstract：
Ophthalmomyiasis is the result of fly larvae feeding on the tissues of the eye. Commonly associated with poor hygiene and open wounds, this condition is rare and often stigmatized. Treatment can be straightforward, and full recovery is common. Identifying the species responsible for ophthalmomyiasis is important for the medical, forensic, and entomological communities. Here, we present a case of ophthalmomyiasis where 30-40 blow fly (Diptera: Calliphoridae) larvae were removed from the eye of a human male. A representative subsample of five larvae was used for taxonomic identification via two approaches (a) DNA analysis, via sequencing of the complete mitochondrial genome (mtGenome) and comparison of the mtGenome and mitochondrial COI barcode region to GenBank, and (b) morphology, examination of the posterior spiracles using microscopy, and comparison to published larval descriptions of blow flies. Two species of blow flies were identified from the DNA analysis: Lucilia coeruleiviridis and Phormia regina. Morphological examination could only confirm L. coeruleiviridis as being present. To our knowledge, finding two blow fly species causing ophthalmomyiasis in a single individual has not been previously reported in the scientific literature. Neither P. regina nor L. coeruleiviridis prefers living tissue for larva development, but since they fill similar ecological niches, perhaps this was a show of competition rather than a normal feeding habit. Knowing these blow fly species can resort to this behavior, and that it can affect human populations, is valuable to the education of patients and providers.
摘要：
眼病是苍蝇幼虫取食眼睛组织的结果。通常与卫生条件差和开放性伤口有关，这种情况很少见，经常受到污名化。治疗可以很简单，完全恢复是常见的。确定导致眼霉菌病的物种对医学很重要，法医,和昆虫学社区。这里,我们介绍了一例眼霉菌病，其中从人类男性的眼睛中取出了30-40只吹蝇（双翅目：Calliphoridae）幼虫。通过两种方法（a）DNA分析，将五个幼虫的代表性子样本用于分类学鉴定，通过对完整的线粒体基因组（mtGenome）进行测序，并将mtGenome和线粒体COI条形码区域与GenBank进行比较，和(B)形态学，使用显微镜检查后的螺旋，并与已发表的苍蝇幼虫描述进行比较。从DNA分析中鉴定出两种蝇：Luciliacoruleviridis和Phormiaregina。形态学检查只能确认存在。据我们所知,以前在科学文献中没有报道过在单个个体中发现两种导致眼霉菌病的吹蝇物种。无论是P.regina还是L.coeruleviridis都不喜欢幼虫发育的活组织，但是由于它们填充了类似的生态位，也许这是竞争的表现，而不是正常的喂养习惯。知道这些苍蝇物种可以诉诸这种行为，它可以影响人类，对患者和提供者的教育很有价值。
